Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines

  • Maria V. Camarasa
  • Robbie W. Kerr
  • Sharon F. Sneddon
  • Nicola Bates
  • Lisa Shaw
  • Rachel A. Oldershaw
  • Fiona Small
  • Melissa A. Baxter
  • Tristan R. Mckay
  • Daniel R. Brison
  • Susan J. Kimber
Article

DOI: 10.1007/s11626-010-9291-5

Cite this article as:
Camarasa, M.V., Kerr, R.W., Sneddon, S.F. et al. In Vitro Cell.Dev.Biol.-Animal (2010) 46: 386. doi:10.1007/s11626-010-9291-5

Abstract

We report here the derivation of two new human embryonic stem cell lines, Man-1 and Man-2, and their full characterization as novel pluripotent stem cell lines. Man-1 was derived from an embryo surplus to requirement from routine IVF, while Man-2 was obtained from an oocyte classified as failed to fertilise and subsequently chemically activated. We report the characterisation of pluripotency and the differentiation potential of these lines. Work is in progress to establish novel methods of stem cell derivation and culture, which will avoid the use of xenobiotics and be relevant to clinical production of human embryonic stem cell lines. Both newly derived human embryonic stem cell lines will be available for the research community from the UK Stem Cell Bank (http://www.ukstemcellbank.org.uk).

Keywords

Stem cells Derivation Research grade 

Copyright information

© The Society for In Vitro Biology 2010

Authors and Affiliations

  • Maria V. Camarasa
    • 1
    • 4
  • Robbie W. Kerr
    • 1
    • 2
  • Sharon F. Sneddon
    • 2
  • Nicola Bates
    • 1
  • Lisa Shaw
    • 2
  • Rachel A. Oldershaw
    • 1
  • Fiona Small
    • 3
  • Melissa A. Baxter
    • 1
  • Tristan R. Mckay
    • 1
  • Daniel R. Brison
    • 1
    • 2
  • Susan J. Kimber
    • 1
  1. 1.North West Embryonic Stem Cell Centre, Faculty of Life SciencesUniversity of ManchesterManchesterUK
  2. 2.North West Embryonic Stem Cell Centre, University of Manchester, Manchester Academic Health Science Centre, Central Manchester University Hospitals NHS Foundation TrustSt Mary’s HospitalManchesterUK
  3. 3.North West Regional Cytogenetics Unit, St Mary’s Hospital, Manchester Royal InfirmaryCentral Manchester University Hospitals NHS Foundation TrustManchesterUK
  4. 4.Development and Regeneration Program, Caubet-Cimera Fundation, Advanced Respiratory Medicine CentreJoan March HospitalPalma de MallorcaSpain

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