Activation of CD74 inhibits migration of human mesenchymal stem cells

  • Bonnie L. Barrilleaux
  • Benjamin W. Fischer-Valuck
  • Jennifer K. Gilliam
  • Donald G. Phinney
  • Kim C. O’Connor
Article
First Online:
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Accepted:

Abstract

Therapeutic administration of mesenchymal stem cells (MSCs) by systemic delivery utilizes the innate ability of the cells to home to damaged tissues, but it can be an inefficient process due to a limited knowledge of cellular cues that regulate migration and homing. Our lab recently discovered that a potent pro-inflammatory cytokine, macrophage migration inhibitory factor (MIF), inhibits MSC migration. Because MIF may act on multiple cellular targets, an activating antibody (CD74Ab) was employed in this study to examine the effect of one MIF receptor, CD74 (major histocompatibility complex class II-associated invariant chain), on MSC motility. CD74 activation inhibits in a dose-dependent manner up to 90% of in vitro migration of MSCs at 40 μg/ml CD74Ab (p < 0.001), with consistent effects observed among three MSC donor preparations. A blocking peptide from the C-terminus of CD74 eliminates the effect of CD74Ab on MSCs. This suggests that MIF may act on MSCs, at least in part, through CD74. Late-passage MSCs exhibit less chemokinesis than those at passage 2. However, MSCs remain responsive to CD74 activation during ex vivo expansion: MSC migration is inhibited ∼2-fold in the presence of 5 µg/ml CD74Ab at passage 9 vs. ∼3-fold at passage 2 (p < 0.001). Consistent with this result, there were no significant differences in CD74 expression at all tested passages or after CD74Ab exposure. Targeting CD74 to regulate migration and homing potentially may be a useful strategy to improve the efficacy of a variety of MSC therapies, including those that require ex vivo expansion.

Keywords

Mesenchymal stem cells CD74 Major histocompatibility complex class II-associated invariant chain Chemokinesis Migration 
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Notes

Acknowledgments

This work was supported by a grant from the National Science Foundation (BES-0514242). Prof. Kim O’Connor is a member of the Tulane Center for Gene Therapy. We thank Andrew Katz for his technical assistance.

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Copyright information

© The Society for In Vitro Biology 2010

Authors and Affiliations

  • Bonnie L. Barrilleaux
    • 1
    • 3
  • Benjamin W. Fischer-Valuck
    • 1
    • 4
  • Jennifer K. Gilliam
    • 1
  • Donald G. Phinney
    • 2
  • Kim C. O’Connor
    • 1
  1. 1.Department of Chemical and Biomolecular EngineeringTulane University, Lindy Boggs Center Room 300New OrleansUSA
  2. 2.Department of Molecular TherapeuticsThe Scripps Research InstituteJupiterUSA
  3. 3.Institute of Pediatric Regenerative MedicineShriners Hospital for ChildrenSacramentoUSA
  4. 4.School of MedicineLouisiana State University Health Sciences CenterShreveportUSA

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