In Vitro Cellular & Developmental Biology - Animal

, Volume 45, Issue 8, pp 467–472 | Cite as

Mechanical loading promotes Lewis lung cancer cell growth through periostin

  • Dedong Ma
  • Hongxiu Lu
  • Lisheng Xu
  • Xia Xu
  • Wei XiaoEmail author


Mechanical loading is known to trigger proliferation of tumor cells. Periostin is a new molecule found to increase in many cancers. To determine how mechanical strain modulates tumor growth and its possible mediation by periostin through TGF-β1, Lewis lung cancer cells were cultured on flexible-bottomed culture plates and cyclically strained using Flexercell Strain Unit. Real-time RT-PCR was used to quantify periostin and TGF-β1 mRNA levels at 6, 12, 18, and 24 h of loading. In addition, periostin and TGF-β1 neutralizing antibodies were added to the medium. We showed that the proliferative ability of Lewis cancer cells was significantly increased by cyclical strain. This change can be blocked by 5 µg/ml of periostin neutralizing antibody. Periostin mRNA increased by 1.1-, 3.2-, 4.7-, and 9.2-fold while TGF-β1 mRNA increased by 5.3-, 10.3-, 7.1-, and 6.5-fold at 6, 12, 18, and 24 h, respectively. Periostin protein in medium increased after cyclical strain. Expression of periostin mRNA in response to mechanical loading was completely blocked by 2.5 µg/ml of TGF-β1 neutralizing antibody. In addition, overexpression of periostin in Lewis cells can promote cell proliferation. Our results suggest that periostin is a potent positive regulator of tumor growth in response to mechanical loading and is possibly a downstream factor of TGF-β1.


Lewis Lung Carcinoma Mechanical stress Periostin Cell proliferation Apoptosis 



The authors acknowledge the technical assistance of Dr. Shiva Kotha with the Flexercell FX-4000 unit. We also acknowledge the assistance of Dr. Jerry Feng and his colleagues. This work was supported by Shandong Gongguan project 2006GG3202043.


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Copyright information

© The Society for In Vitro Biology 2009

Authors and Affiliations

  • Dedong Ma
    • 1
    • 2
  • Hongxiu Lu
    • 3
  • Lisheng Xu
    • 1
  • Xia Xu
    • 1
  • Wei Xiao
    • 1
    Email author
  1. 1.Department Respiratory Medicine, Qilu HospitalShandong UniversityJinanChina
  2. 2.Department Oral biologyUniversity of Missouri-Kansas CityKansas CityUSA
  3. 3.Department AnesthesiologyAffiliated Hospital of Shandong University of traditional Chinese MedicineJinanChina

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