Decreased surfactant protein B expression in mice derived completely from embryonic stem cells
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ES mice that are derived completely from embryonic stem (ES) cells can be obtained by tetraploid embryo complementation. Many neonate ES mice die because of respiratory distress, but it is not clear what contributes to the phenomenon. Using five microsatellite DNA markers, we confirmed that our ES mice were completely derived from ES cells and contained no tetraploid component. The neonatal ES mice that exhibited respiratory distress were tested for surfactant protein B (SP-B) expression by Western blotting. These mice had no SP-B expression, and even apparently healthy adult ES mice had decreased SP-B levels and aberrant SP-B phenotypes. These data suggest that the expression of SP-B protein is an important factor in the survival of ES mice to term and adulthood.
KeywordsES mice Respiratory distress Pulmonary surfactant protein B Tetraploid embryo complementation
We thank Prof. Zhijie Chang for kindly providing the BCATM Protein Assay Kit (PIERCE) and assisting in the Western blotting. We also thank Dr. Man Hu, Dr. Yuzhong Ma, Mrs. Xianrui Gu, Mrs. Rongyan Zhou, Mr. Jie Tang, and Mr. Lei Ju for the many suggestions and help. In particular, we would like to thanks Mrs. Eileen Rattner for her critical remarks and editing this manuscript. This work was supported by the National Natural Science Foundation of China (No.30571336) and the President Foundation from Agricultural University of Hebei.
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