CFTR IVS8 Poly-T Variation Affects Severity of Acute Pancreatitis in Women
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Cystic fibrosis transmembrane conductance regulator (CFTR) is important for normal pancreatic function. Its coding gene is polymorphic, and the variations have been associated with the increased risk for acute pancreatitis. However, their impact on the disease severity is still unknown. Therefore, the aim of our study was to determine the functional importance of common cystic fibrosis transmembrane conductance regulator variations IVS8-poly T, R117H, and M470V for the severity of acute pancreatitis.
The study involved 98 acute pancreatitis patients. The severity of the disease was determined based on the Atlanta Classification system. IVS8-poly T, R117H, and M470V genotyping was performed using PCR-RFLP method.
IVS8-5T, IVS8-7T, IVS8-9T, and M470V alleles were found at the frequencies of 5.7, 75.5, 18.9, and 55.7%, respectively, while R117H was not observed. Among women, the severe form of the disease was more frequent in carriers of at least one IVS8 9T allele (RR for 9T/9T + 9T/non-9T vs. non-9T/non-9T: 2.115; 95% CI: 1.241–3.605). This association was not detected in men and was not affected by M470V. In addition, co-morbidities increased the severity of acute pancreatitis (p = 0.022).
Our study reveals that IVS8 poly-T variation affects severity of acute pancreatitis in women and that existent co-morbidities worsen the clinical course of the disease.
KeywordsAcute pancreatitis CFTR IVS8-poly T Sex
Ivan Radosavljevic contributed acquisition of data, interpretation of data, and drafting of the manuscript. Bojan Stojanovic and Marko Spasic contributed acquisition of data. Slobodan Jankovic contributed study concept and design and interpretation of the data. Natasa Djordjevic contributed study concept and design, statistical analysis, interpretation of the data, and drafting of the manuscript. All authors contributed to critical manuscript revision.
The study was financially supported by the Faculty of Medical Sciences, University of Kragujevac, Serbia, JP 10/11, and the Ministry of Science and Technological Development, Republic of Serbia, grants No. 175007 and 175056.
Compliance with Ethical Standards
The study was conducted in accordance with the Declaration of Helsinki and its subsequent revisions, with all the patients giving their written informed consent. The study was approved on August 29th, 2011, by the ethics committee at the Clinical Centre Kragujevac, decision No 01-9024.
Conflict of Interest
The authors declare that they have no conflict of interest.
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