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Journal of Gastrointestinal Surgery

, Volume 16, Issue 6, pp 1136–1143 | Cite as

Genomic Analysis of Pterostilbene Predicts Its Antiproliferative Effects Against Pancreatic Cancer In Vitro and In Vivo

  • Denise Elizabeth McCormack
  • Patrick Mannal
  • Debbie McDonald
  • Scott Tighe
  • Joshua Hanson
  • David McFadden
2011 SSAT Poster Presentation

Abstract

Background

To investigate the inhibitory role of pterostilbene in pancreatic cancer, we conducted a genomic analysis of pterostilbene-treated pancreatic cancer cells. We also investigated the effect of pterostilbene upon the carcinogenic markers, manganese superoxide dismutase, cytochrome C, Smac/DIABLO, and STAT3 phosphorylation in vitro. The antiproliferative effects of pterostilbene were further evaluated in an in vivo model.

Methods

Pancreatic cancer cells were treated with pterostilbene and evaluated with DNA microarray analysis. Pterostilbene-treated cells were analyzed for cytochrome C, Smac/DIABLO, manganese superoxide dismutase (MnSOD)/antioxidant activity, and STAT3 phosphorylation using ELISA. Data were statistically analyzed using ANOVA. Pterostilbene was then administered to nude mice for 8 weeks, and tumor growth rates were recorded and statistically analyzed.

Results

Microarray analysis of pterostilbene-treated cells revealed upregulation of pro-apoptosis genes. In vitro, pterostilbene treatment altered levels of phosphorylated STAT3, MnSOD/antioxidant activity, cytochrome C, and Smac/DIABLO. In nude mice, oral pterostilbene inhibited tumor growth rates.

Conclusion

Pterostilbene alters gene expression in pancreatic cancer and increases the antiproliferative markers cytochrome C, Smac/DIABLO, and MnSOD/antioxidant activity. It was also shown to inhibit phosphorylated STAT3, a marker of accelerated tumorigenesis, and decrease pancreatic tumor growth in vivo. Further studies are warranted to elucidate the effects of pterostilbene in humans.

Keywords

Pancreatic cancer Pterostilbene Apoptosis 

Notes

Acknowledgments

Microarray analysis was conducted by the Vermont Genetics Network microarray facility through grant number 2P20RR016462 from the INBRE Program of the National Center for Research Resources (NCRR). Bioinformatic support and data analysis was conducted by Dr. Jeff Bond at the University of Vermont Bioinformatics shared resource facility.

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Copyright information

© The Society for Surgery of the Alimentary Tract 2012

Authors and Affiliations

  • Denise Elizabeth McCormack
    • 1
  • Patrick Mannal
    • 2
  • Debbie McDonald
    • 3
  • Scott Tighe
    • 4
  • Joshua Hanson
    • 5
  • David McFadden
    • 6
  1. 1.Department of SurgeryDanbury HospitalDanburyUSA
  2. 2.Department of Surgery, Fletcher Allen Health CareUniversity of VermontBurlingtonUSA
  3. 3.Department of Surgery, Fletcher Allen Health CareUniversity of VermontBurlingtonUSA
  4. 4.Advanced Genome Technology LabUniversity of VermontBurlingtonUSA
  5. 5.Department of Pathology, Fletcher Allen Health CareUniversity of VermontBurlingtonUSA
  6. 6.University of Connecticut Health CenterFarmingtonUSA

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