Gastric Cardia Carcinoma is Associated with the Promoter -77T>C Gene Polymorphism of X-Ray Cross-Complementing Group 1 (XRCC1)

  • Giovanni Corso
  • Daniele Marrelli
  • Corrado Pedrazzani
  • José Carlos Machado
  • Stefano Mancini
  • Raquel Seruca
  • Franco Roviello
Original Article
First Online:
Received:
Accepted:

Abstract

Purpose

X-ray repair cross complementing group 1 (XRCC1) is one of the major DNA repair proteins involved in the bas-excision repair pathway. Several single-nucleotide polymorphisms in the XRCC1 gene are identified and related with increased cancer risk development. In particular, the -77T>C polymorphism located on the promoter region relates with lung cancer risk development. The aim of this study is to analyze the -77T>C allelic frequencies in a population composed of 456 primary gastric cancer patients (GC) and 507 blood donor controls.

Methods

GC patients were observed at the University of Siena, Italy; clinicopathological data and family history were available for the cancer group. The control group is composed of blood donors. Constitutional genomic DNA was PCR amplified, and XRCC1 -77T>C was detected using restriction enzyme BsrB I and analyzed in a 3% agarose gel.

Results

The -77C>C homozygous genotype was significantly associated with increased risk of gastric cardia carcinoma (p = 0.023) with an odds ratio of 1.65 (95% confidence interval 1.14 to 2.4). In the family history stratification, we report a significant association (p = 0.043) between the -77T>C polymorphism and GC cases with familial lung cancer aggregation.

Conclusions

Our results suggest that the XRCC1 -77T>C polymorphism is a relevant host susceptibility factor for gastric cardia cancer development and specific subsets of familial clustering of GC.

Keywords

Gastric cancer Single nucleotide genetic polymorphism Risk factor 
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Notes

Acknowledgments

This work was supported by Istituto Toscano Tumori (“Gene expression profile and therapeutic implication in gastric cancer. From the clinical overview to the translational research”; Grant ITT-2007) and by Fundação para a Ciência e a Tecnologia (“Exploring the role of E-Cadherin-HER interaction in the search of molecular biomarkers for the clinical management of gastric cancer patients”; grant ref PIC/IC/82923/2007 and PhD grant ref SFRH/BD/40090/2007).

Competing interests

The author(s) declare that they have no competing interests.

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Copyright information

© The Society for Surgery of the Alimentary Tract 2009

Authors and Affiliations

  • Giovanni Corso
    • 1
    • 2
    • 3
    • 6
  • Daniele Marrelli
    • 1
    • 3
  • Corrado Pedrazzani
    • 1
    • 3
  • José Carlos Machado
    • 2
    • 4
  • Stefano Mancini
    • 5
  • Raquel Seruca
    • 2
    • 4
  • Franco Roviello
    • 1
    • 3
  1. 1.Department of Human Pathology and Oncology, Section of General Surgery and Surgical Oncology, Translational Research LaboratoryUniversity of SienaSienaItaly
  2. 2.Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP)PortoPortugal
  3. 3.Istituto Toscano TumoriFlorenceItaly
  4. 4.Faculdade de Medicina da Universidade do PortoPortoPortugal
  5. 5.Department of SurgeryUniversity of SienaSienaItaly
  6. 6.Policlinico le ScotteSienaItaly

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