MicroRNA-21 is Overexpressed in Pancreatic Cancer and a Potential Predictor of Survival

  • Mary Dillhoff
  • James Liu
  • Wendy Frankel
  • Carlo Croce
  • Mark Bloomston
original paper



MicroRNAs are small (18–22 nucleotides) noncoding RNAs involved in posttranscriptional modification of many target genes. One of these, microRNA-21 (miR-21), has been shown to play a role in multiple hematologic and solid organ malignancies. We sought to determine the expression pattern of miR-21 in pancreatic cancers and its impact on clinicopathologic characteristics.


Eighty resected pancreatic cancer specimens were microdissected and tissue microarrays (TMA) created in duplicate. TMAs were also created for benign pancreas (N = 12) and chronic pancreatitis (N = 45). In situ hybridization (ISH) was undertaken utilizing locked nucleic acid probes for miR-21. RNA U6 and scrambled RNA served as positive and negative control, respectively. ISH was scored as 0 (absent), 1+ (faint/focal expression), or 2+ (strong expression). Kaplan–Meier survival curves were constructed and compared by log-rank analysis.


MiR-21 expression was demonstrated in 63 (79%) pancreatic cancers (1+ in 49, 2+ in 14) compared to one of 12 (8%, p < 0.0001) benign pancreas and 12/45 (27%, p < 0.0001) chronic pancreatitis. None of the benign tissues demonstrated strong miR-21 expression. Although miR-21 expression did not correlate with tumor size, differentiation, nodal status, or T stage, strong miR-21 expression was predictive of poorer outcome compared to absent or faint/focal miR-21 expression in patients with node-negative disease (median 27.7 months vs. 15.2, p = 0.037). Nodal status was also predictive of survival (p = 0.029).


MicroRNA-21 is significantly overexpressed in pancreatic cancers as detected by in situ hybridization. Its strong expression predicts limited survival in patients with node-negative disease and may be an important biologic marker for outcome.


MicroRNA MiRNA MiR-21 Pancreatic cancer 


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Copyright information

© The Society for Surgery of the Alimentary Tract 2008

Authors and Affiliations

  • Mary Dillhoff
    • 1
  • James Liu
    • 2
  • Wendy Frankel
    • 2
  • Carlo Croce
    • 3
  • Mark Bloomston
    • 1
  1. 1.Department of SurgeryThe Ohio State UniversityColumbusUSA
  2. 2.Department of PathologyThe Ohio State UniversityColumbusUSA
  3. 3.Department of Molecular Virology, Immunology and Molecular GeneticsThe Ohio State UniversityColumbusUSA

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