Skip to main content

Advertisement

SpringerLink
Log in

Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits

  • Original article
  • Published:
Japanese Journal of Radiology Aims and scope Submit manuscript

Abstract

Purpose

To determine cellular viability of lung parenchyma and neoplastic cells in areas of ground-glass opacity (GGO) on computed tomography (CT) images immediately after pulmonary radiofrequency ablation (RFA) in rabbits.

Materials and methods

A LeVeen RFA electrode was placed percutaneously into rabbit lungs with or without metastatic VX2 tumors. Five minutes later, seven isolated lungs were imaged by use of a multi-detector row CT scanner, and the images were compared with histological features. The cellular viability of the lung tissues was assessed by nicotinamide adenine dinucleotide hydrogen (NADH) staining in eight normal lungs and in three lungs with multiple metastatic tumors.

Results

All lung lesions appeared as bilayered structures with a central, dense, attenuated area and an outer area of GGO on CT images, and as three-layered structures on macroscopic and microscopic images 5 min after RFA. The GGO areas approximately corresponded to the outer two layers in macroscopic images that were exudative and congestive on microscopic images. Staining for NADH was significantly reduced in the GGO and densely attenuated areas with or without tumor tissue staining compared with the non-ablated area.

Conclusions

Our results suggest that an area of GGO that appears on CT immediately after RFA can be effectively treated by RFA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Fry WA, Phillips JL, Menck HR. Ten-year survey of lung cancer treatment and survival in hospitals in the United States: a national cancer data base report. Cancer. 1999;86:1867–76.

    Article  PubMed  CAS  Google Scholar 

  2. Simon CJ, Dupuy DE, DiPetrillo TA, Safran HP, Grieco CA, Ng T, et al. Pulmonary radiofrequency ablation: long-term safety and efficacy in 153 patients. Radiology. 2007;243:268–75.

    Article  PubMed  Google Scholar 

  3. Gazelle GS, Goldberg SN, Solbiati L, Livraghi T. Tumor ablation with radio-frequency energy. Radiology. 2000;217:633–46.

    PubMed  CAS  Google Scholar 

  4. Kelekis AD, Thanos L, Mylona S, Ptohis N, Malaqari K, Nikita A, et al. Percutaneous radiofrequency ablation of lung tumors with expandable needle electrodes: current status. Eur Radiol. 2006;16:2471–82.

    Article  PubMed  CAS  Google Scholar 

  5. Wolf FJ, Grand DJ, Machan JT, Dipetrillo TA, Mayo-Smith WW, Dupuy DE. Microwave ablation of lung malignancies: effectiveness, CT findings, and safety in 50 patients. Radiology. 2008;247:871–9.

    Article  PubMed  Google Scholar 

  6. Bojarski JD, Dupuy DE, Mayo-Smith WW. CT imaging findings of pulmonary neoplasms after treatment with radiofrequency ablation: results in 32 tumors. AJR. 2005;185:466–71.

    PubMed  Google Scholar 

  7. Yamamoto A, Nakamura K, Matsuoka T, Toyoshima M, Okuma T, Oyama Y, et al. Radiofrequency ablation in a porcine lung model: correlation between CT and histopathologic findings. AJR. 2005;185:1299–306.

    Article  PubMed  Google Scholar 

  8. Hiraki T, Tajiri N, Mimura H, Yasui K, Gobara H, Mukai T, et al. Pneumothorax, pleural effusion, and chest tube placement after radiofrequency ablation of lung tumors: incidence and risk factors. Radiology. 2006;241:275–83.

    Article  PubMed  Google Scholar 

  9. Neumann RA, Knobler RM, Pieczkowski F, Gebhart W. Enzyme histochemical analysis of cell viability after argon laser-induced coagulation necrosis of the skin. J Am Acad Dermatol. 1991;25:991–8.

    Article  PubMed  CAS  Google Scholar 

  10. Okuma T, Matsuoka T, Okamura T, Wada Y, Yamamoto A, Oyama Y, et al. 18F-FDG small-animal PET for monitoring the therapeutic effect of CT-guided radiofrequency ablation on implanted VX2 tumors in rabbits. J Nucl Med. 2006;47:1351–8.

    PubMed  Google Scholar 

  11. Hyoudou K, Nishikawa M, Umeyama Y, Kobayashi Y, Yamashita F, Hashida M. Inhibition of metastatic tumor growth in mouse lung by repeated administration of polyethylene glycol-conjugated catalase: quantitative analysis with firefly luciferase-expressing melanoma cells. Clin Cancer Res. 2004;10:7685–91.

    Article  PubMed  CAS  Google Scholar 

  12. Yasui K, Kanazawa S, Sano Y, Fujiwara T, Kagawa S, Mimura H, et al. Thoracic tumors treated with CT-guided radiofrequency ablation: initial experience. Radiology. 2004;231:850–7.

    Article  PubMed  Google Scholar 

  13. Goldberg SN, Gazelle GS, Compton CC, McLoud TC. Radiofrequency tissue ablation in the rabbit lung: efficacy and complications. Acad Radiol. 1995;2:776–84.

    Article  PubMed  CAS  Google Scholar 

  14. Miao Y, Ni Y, Bosmans H, Yu J, Vaninbroukx J, Dymarkowski S, et al. Radiofrequency ablation for eradication of pulmonary tumor in rabbits. J Surg Res. 2001;99:265–71.

    Article  PubMed  CAS  Google Scholar 

  15. Lee JM, Jin GY, Li CA, Chung GH, Lee SY, Han YM, et al. Percutaneous radiofrequency thermal ablation of lung VX2 tumors in a rabbit model using a cooled tip-electrode: feasibility, safety, and effectiveness. Invest Radiol. 2003;38:129–39.

    Article  PubMed  Google Scholar 

  16. Anderson EM, Lees WR, Gillams AR. Early indicators of treatment success after percutaneous radiofrequency of pulmonary tumors. Cardiovasc Intervent Radiol. 2009;32:478–83.

    Article  PubMed  Google Scholar 

  17. de Baère T, Palussière J, Aupérin A, Hakime A, Abdel-Rehim M, Kind M, et al. Midterm local efficacy and survival after radiofrequency ablation of lung tumors with minimum follow-up of 1 year: prospective evaluation. Radiology. 2006;240:587–96.

    Article  PubMed  Google Scholar 

  18. Michaels MJ, Rhee HK, Mourtzinos AP, Summerhayes IC, Silverman ML, Libertino JA. Incomplete renal tumor destruction using radio frequency interstitial ablation. J Urol. 2002;168:2406–10.

    Article  PubMed  Google Scholar 

  19. Anderson JK, Baker M, Jaffers O, Pearle MS, Lindberg GL, Cadeddu JA. Time course of nicotinamide adenine dinucleotide diaphorase staining after renal radiofrequency ablation influence viability assessment. J Endourol. 2007;21:223–7.

    Article  PubMed  Google Scholar 

  20. Stern JM, Anderson JK, Lotan Y, Park S, Cadeddu JA. Nicotinamide adenine dinucleotide staining immediately following radio frequency ablation of renal tumors—is a positive stain synonymous with ablative failure? J Urol. 2006;176:1969–72.

    Article  PubMed  Google Scholar 

  21. Goldberg SN, Hahn PF, Tanabe KK, Mueller PR, Schima W, Athanasoulis CA, et al. Percutaneous radiofrequency tissue ablation: does perfusion-mediated tissue cooling limit coagulation necrosis? J Vasc Interv Radiol. 1998;9:101–11.

    Article  PubMed  CAS  Google Scholar 

  22. Yan TD, King J, Sjarif A, Glenn D, Steinke K, Morris DL. Percutaneous radiofrequency ablation of pulmonary metastases from colorectal carcinoma: prognostic determinants for survival. Ann Surg Oncol. 2006;13:1529–37.

    Article  PubMed  Google Scholar 

  23. Lencioni R, Crocetti L, Cioni R, Suh R, Glenn D, Regge D, et al. Response to radiofrequency ablation of pulmonary tumours: a prospective, intention-to-treat, multicentre clinical trial (the RAPTURE study). Lancet Oncol. 2008;9:621–8.

    Article  PubMed  Google Scholar 

  24. Beland MD, Wasser EJ, Mayo-Smith WW, Dupuy DE. Primary non-small cell lung cancer: review of frequency, location, and time of recurrence after radiofrequency ablation. Radiology. 2010;254:301–7.

    PubMed  Google Scholar 

  25. Dupuy DE. Image-guided thermal ablation of lung malignancies. Radiology. 2011;260:633–55.

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

We are deeply grateful to Ms Kyoko Ohashi for technical assistance.

Author information

Authors and Affiliations

  1. Department of Radiology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Masaomi Kuroki, Hiroshi Nakada, Noriko Uchino, Shinya Sato & Shozo Tamura

  2. Department of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

    Atsushi Yamashita & Yujiro Asada

  3. Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan

    Akira Sawaguchi

  4. Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan

    Taketoshi Asanuma

Authors
  1. Masaomi Kuroki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hiroshi Nakada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Atsushi Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akira Sawaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Noriko Uchino
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shinya Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Taketoshi Asanuma
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yujiro Asada
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Shozo Tamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masaomi Kuroki.

About this article

Cite this article

Kuroki, M., Nakada, H., Yamashita, A. et al. Loss of cellular viability in areas of ground-glass opacity on computed tomography images immediately after pulmonary radiofrequency ablation in rabbits. Jpn J Radiol 30, 323–330 (2012). https://doi.org/10.1007/s11604-012-0054-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11604-012-0054-y

Keywords

Search

Navigation