Investigation of protective effect of hydrogen-rich water against cisplatininduced nephrotoxicity in rats using blood oxygenation level-dependent magnetic resonance imaging

  • Taro Matsushita
  • Yoshinori Kusakabe
  • Akihiro Kitamura
  • Sakie Okada
  • Kenya Murase
Original Article



The aim of this study was to assess the mechanism of the protective effect of hydrogen-rich water (HW) against cisplatin (CP)-induced nephrotoxicity in rats using blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI).

Materials and methods

Apparent transverse relaxation time-weighted images (T2*WI) were acquired in 28 rats. The control group (n = 7) had free access to standard water (SW) and no CP injection. The CP group (n = 7) had free access to SW and was given a CP injection on day 0. The CP+HW group (n = 7) had free access to HW and had a CP injection. The HW group (n = 7) had free access to HW and no CP injection. The apparent transverse relaxation rate (R2*) was estimated from T2*WI.


In the CP+HW group, the R2* value in the medulla normalized by the value of the day 0 was significantly greater than that in the CP group on days 4 and 7. The creatinine and blood urea nitrogen levels in the CP group were significantly higher than those in the control, CP+HW, and HW groups.


BOLD MRI may be useful for demonstrating the change in R2* in CP-induced nephrotoxicity in rats. The changes in the CP+HW group were suspected to be due to a reduction of cytotoxic oxygen radicals.

Key words

Blood oxygenation level-dependent MRI Cisplatin-induced nephrotoxicity Hydrogen-rich water Oxygen consumption Rats 


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Copyright information

© Japan Radiological Society 2011

Authors and Affiliations

  • Taro Matsushita
    • 1
  • Yoshinori Kusakabe
    • 1
  • Akihiro Kitamura
    • 1
  • Sakie Okada
    • 1
  • Kenya Murase
    • 1
  1. 1.Department of Medical Physics and Engineering, Division of Medical Technology and Science, Faculty of Health Science, Graduate School of MedicineOsaka UniversitySuita, OsakaJapan

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