Japanese Journal of Radiology

, Volume 28, Issue 2, pp 173–179 | Cite as

Selection of endogenous 13C substrates for observation of intracellular metabolism using the dynamic nuclear polarization technique

  • Masafumi Harada
  • Hitoshi Kubo
  • Takamasa Abe
  • Hiroshi Maezawa
  • Hideki Otsuka
Technical Note

Abstract

Purpose

The aim of this study was to select a suitable substrate candidate for dynamic nuclear polarization (DNP) studies and demonstrate its utility for evaluating intracellular metabolism.

Materials and methods

Hyperpolarized substances included 1-13C-pyruvate (Pyr), 1-13C-glucose (Glc), and 1-13C-acetate. A DNP polarizer and a 600-MHz vertical small-bore scanner were used for 13C-MR spectroscopic measurements. After polarization for 1 h, the dissolved solution was injected via a capillary line into the nuclear magnetic resonance tube in the scanner. The sequential spectra of the hyperpolarized 13C-labeled substrates were acquired in durations of more than 120 s, and a thermal spectrum was obtained more than 1 h thereafter. FM3A cancer cells of mammary tumors were cultured for intracellular detection of the hyperpolarized 13C-substances.

Results

The greatest sensitivity was found using Pyr with the longest T1 decay (51.5 s); and remarkably, the least sensitivity was observed using Glc with a signal decay of less than 2 s. An effective increase in sensitivity was shown using the other substances. The hyperpolarized intracellular study using 13C-Pyr showed distinct elevation of lactate levels.

Conclusion

The DNP technique is useful for evaluating intracellular metabolism. However, Glc is not suitable for use with the DNP technique.

Key words

13C-MRS DNP Pyruvate Glucose Tumor cell 

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Copyright information

© Japan Radiological Society 2010

Authors and Affiliations

  • Masafumi Harada
    • 1
  • Hitoshi Kubo
    • 1
  • Takamasa Abe
    • 2
  • Hiroshi Maezawa
    • 3
  • Hideki Otsuka
    • 4
  1. 1.Department of Medical Imaging, Institute of Health BiosciencesUniversity of Tokushima Graduate SchoolTokushimaJapan
  2. 2.MRI/Biotools DivisionOxford Instruments KKTokyoJapan
  3. 3.Department of Radiation Physics, Engineering, and Biology, Institute of Health BiosciencesUniversity of Tokushima Graduate SchoolTokushimaJapan
  4. 4.Department of Radiology, Institute of Health BiosciencesUniversity of TokushimaTokushimaJapan

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