Current Medical Science

, Volume 38, Issue 6, pp 1045–1053 | Cite as

Essential Oil from Carpesium abrotanoides L. Induces Apoptosis via Activating Mitochondrial Pathway in Hepatocellular Carcinoma Cells

  • Qiang Wang
  • Li-hong Pan
  • Li Lin
  • Ren Zhang
  • Yu-chao Du
  • Hao Chen
  • Mi Huang
  • Kai-wen GuoEmail author
  • Xin-zhou YangEmail author


The effects of essential oil from Carpesium abrotanoides L. (CAEO) on the proliferation and apoptosis of human hepatic cancer cells were investigated in this study. MTT assays indicated that CAEO inhibited the proliferation of HCC cells with the IC50 values ranging from 41.28±3.06 to 130.36±20.79 μg/mL. Moreover, many obviously nuclear morphological changes of apoptotic cells in CAEO-treated HepG2 cells were detected by Hoechst 33258 staining and fluorescence microscopy. Flow cytometry was used to detect cell apoptosis and cell cycle, and noticeable findings showed that CAEO arrested cell-cycle at S and G2/M phases. The decreased Bcl-2/Bax protein ratio and the activation of caspase-3, caspase-9 were also detected by Western blotting. All results suggested that CAEO is a potential agent to fight against liver cancer, and the mitochondria-mediated intrinsic apoptotic pathway could be involved in CAEO-mediated apoptosis of human liver carcinoma cells.

Key words

Carpesium abrotanoides L. essential oil HepG2 apoptosis mitochondrial pathway 


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  1. 1.
    Wang XK, Peng Y, Tao HR, et al. Inhibition of adhesion and metastasis of HepG2 hepatocellular carcinoma cells in vitro by DNA aptamer against Sialyl Lewis X. J Huazhong Univ Sci Technolog Med Sci, 2017,37(3):343–347CrossRefGoogle Scholar
  2. 2.
    Befeler AS, Di Bisceglie AM. Hepatocellular carcinoma: diagnosis and treatment. Gastroenterology, 2002,122(6):1609–1619CrossRefGoogle Scholar
  3. 3.
    Gao Y, Chen Y, Ji X, et al. Controlled intracellular release of doxorubicin in multidrug-resistant cancer cells by tuning the shell-pore sizes of mesoporous silica nanoparticles. ACS Nano, 2011,5(12):9788–9798CrossRefGoogle Scholar
  4. 4.
    Hu Y, Wang S, Wu X, et al. Chinese herbal medicinederived compounds for cancer therapy: a focus on hepatocellular carcinoma. J Ethnopharmacol, 2013,149(3):601–612CrossRefGoogle Scholar
  5. 5.
    Nobili S, Lippi D, Witort E, et al. Natural compounds for cancer treatment and prevention. Pharmacol Res, 2009,59(6):365–378CrossRefGoogle Scholar
  6. 6.
    Wang JF, He WJ, Zhang XX, et al. Dicarabrol, a new dimeric sesquiterpene from Carpesium abrotanoides L. Bioorg Med Chem Lett, 2015,25(19):4082–4084CrossRefGoogle Scholar
  7. 7.
    Liu PA, Liu M, Pan WW, et al. Study on chemical constituents from Carpesium abrotanoides. J Chin Med Mater, 2014,37(4):2213–2215Google Scholar
  8. 8.
    Lee SB, Kang K, Lee HJ, et al. The chemopreventive effects of Carpesium abrotanoides are mediated by induction of phase II detoxification enzymes and apoptosis in human colorectal cancer cells. J Med Food, 2010,13(1):39–46CrossRefGoogle Scholar
  9. 9.
    Shen BB, Tang XY, Chen L, et al. Analysis of volatile constituents from aerial part of Carpesium abrotanoides by SPME-GC/MS. Mod Chin Med, 2017,19(7):965–971Google Scholar
  10. 10.
    Lv D, Guo K, Xu C, et al. Essential oil from Siegesbeckia pubescens induces apoptosis through the mitochondrial pathway in human HepG2 cells. J Huazhong Univ Sci Technolog Med Sci, 2017,37(1):87–92CrossRefGoogle Scholar
  11. 11.
    Edinger AL, Thompson CB. Death by design: apoptosis, necrosis and autophagy. Curr Opin Cell Biol, 2004,16(6):663–669CrossRefGoogle Scholar
  12. 12.
    Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocr Rev, 1993,14(2):133–151Google Scholar
  13. 13.
    Peltomaki P. Role of DNA mismatch repair defects in the pathogenesis of human cancer. J Clin Oncol, 2003,21(6):1174–1179CrossRefGoogle Scholar
  14. 14.
    Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation. Cell, 2011, 144 (5):646–674CrossRefGoogle Scholar
  15. 15.
    Häcker G. The morphology of apoptosis. Cell Tissue Res, 2000,301(1):5–17CrossRefGoogle Scholar
  16. 16.
    Evan GI, Vousden KH. Proliferation, cell cycle and apoptosis in cancer. Nature, 2001, 411 (6835):342–348CrossRefGoogle Scholar
  17. 17.
    Wu BX, Zeidan YH, Hannun YA. Downregulation of neutral ceramidase by gemcitabine: Implications for cell cycle regulation. Biochim Biophys Acta, 2009,1791(8):730–739CrossRefGoogle Scholar
  18. 18.
    Bentele M, Lavrik I, Ulrich M, et al. Mathematical modeling reveals threshold mechanism in CD95-induced apoptosis. J Cell Biol, 2004,166(6):839–851CrossRefGoogle Scholar
  19. 19.
    Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol, 2007,35(6):495–516CrossRefGoogle Scholar
  20. 20.
    Takahashi A, Masuda A, Sun M, et al. Oxidative stressinduced apoptosis is associated with alterations in mitochondrial caspase activity and Bcl-2-dependent alterations in mitochondrial pH. Brain Res Bull, 2004,62(6):497–504CrossRefGoogle Scholar
  21. 21.
    Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science, 1998,281(5381):1322–1326CrossRefGoogle Scholar
  22. 22.
    Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell, 1997,91(4):479–489CrossRefGoogle Scholar

Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Qiang Wang
    • 1
  • Li-hong Pan
    • 2
  • Li Lin
    • 1
  • Ren Zhang
    • 1
  • Yu-chao Du
    • 1
  • Hao Chen
    • 3
  • Mi Huang
    • 3
  • Kai-wen Guo
    • 1
    Email author
  • Xin-zhou Yang
    • 3
    Email author
  1. 1.Institute of Infection, Immunology and Tumor Microenvironments, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical CollegeWuhan University of Science and TechnologyWuhanChina
  2. 2.College of Basic Medicine and Forensic Medicine of Hangzhou Medical CollegeHangzhouChina
  3. 3.School of Pharmaceutical SciencesSouth-Central University for NationalitiesWuhanChina

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