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Current Medical Science

, Volume 38, Issue 5, pp 840–846 | Cite as

Inhibitory Effect of LPS on the Proliferation of Oligodendrocyte Precursor Cells through the Notch Signaling Pathway in Intrauterine Infection-induced Rats

  • Yan-qin Ying
  • Xue-qin Yan
  • Sheng-juan Jin
  • Yan Liang
  • Ling Hou
  • Wan-ting Niu
  • Xiao-ping LuoEmail author
Article

Abstract

Periventricular white matter injury (PWMI) is very common in survivors of premature birth, and the final outcomes are a reduction in myelinated neurons leading to white matter hypomyelination. How and (or) why the oligodendrocyte lineage develops abnormally and myelination is reduced is a hot topic in the field. This study focuses on the effect of intrauterine inflammation on the proliferation of oligodendrocyte lineage cells and the underlying mechanisms. Lipopolysaccharide (LPS) (300 μg/kg) was intraperitoneally injected into pregnant Sprague-Dawley rats at embryonic days 19 and 20 to establish a rat model of intrauterine infection-induced white matter injury. Corpus callosum tissues were collected at postnatal day 14 (P14) to quantify the number of oligodendrocytes, the number and proliferation of oligodendrocyte precursor cells (OPCs), and the expression of myelin proteins (MBP and PLP). Furthermore, the expression of Wnt and Notch signaling-related proteins was analyzed. The results showed that the number of oligodendrocytes in the corpus callosum tissues of LPS-treated rats was reduced, and the expression levels of myelinating proteins were down-regulated. Further analysis showed that the Notch signaling pathway was down-regulated in the LPStreated group. These results indicate that intrauterine LPS may inhibit the proliferation of OPCs by down-regulating the Notch rather than the Wnt signaling pathway, leading to hypomyelination of white matter.

Key words

oligodendrocyte precursor cells intrauterine infection hypomyelination lipopolysaccharide signaling pathway 

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Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Yan-qin Ying
    • 1
  • Xue-qin Yan
    • 2
  • Sheng-juan Jin
    • 1
  • Yan Liang
    • 1
  • Ling Hou
    • 1
  • Wan-ting Niu
    • 3
  • Xiao-ping Luo
    • 1
    Email author
  1. 1.Department of Pediatrics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Children HealthcareZhongshan Boai Hospital Affiliated to Southern Medical UniversityZhongshanChina
  3. 3.VA Boston Healthcare System, Department of Orthopedics, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA

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