Current Medical Science

, Volume 38, Issue 4, pp 704–713 | Cite as

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats

  • Wen-ya Huang
  • Xin Zou
  • Fu-er Lu
  • Hao Su
  • Chu Zhang
  • Yan-lin Ren
  • Ke Fang
  • Li-jun Xu
  • Kai-fu Wang
  • Qing-jie Chen
  • Hui DongEmail author


This study aims to explore the effect and mechanism of Jiao-tai-wan (JTW) on systemic and tissue-specific inflammation and insulin resistance in obesity-resistant (OR) rats with chronic partial sleep deprivation (PSD). OR rats with PSD were orally given JTW and Estazolam for 4 weeks. The amount of food intake and metabolic parameters such as body weight increase rate, fasting plasma glucose (FPG), fasting insulin (FINS), homeostasis model assessment-insulin resistance (HOMA-IR) and plasma inflammatory markers were measured. The expression levels of circadian proteins cryptochrome 1 (Cryl) and cryptochrome 2 (Cry2) in hypothalamus, adipose and liver tissues were also determined. Meanwhile, the mRNA expression of inflammatory markers, activity of nuclear factor kappa B (NF-κB) p65 protein, as well as the expression levels of insulin signaling pathway proteins in hypothalamus, adipose and liver tissues were measured. Additionally, cyclic adenosine 3′, 5′-monophosphate (cAMP) and activity of vasodilator-stimulated phosphoprotein (VASP) in hypothalamus tissue were measured. JTW significantly decreased the body weight increase rate and food intake, ameliorated systemic inflammation and insulin resistance. JTW effectively ameliorated inflammation and increased PI3K/AKT signaling activation in hypothalamus, adipose and liver. Interestingly, all these changes were associated with the up-regulation of circadian gene Cryl and Cry2 protein expression. We also found that in hypothalamus tissue of PSD rats, down-regulation of Cryl and Cry2 activated cAMP/PKA signaling and then led to inflammation, while JTW inhibited this signaling. These results suggested that JTW has the beneficial effect on ameliorating inflammation and insulin resistance in partially sleep-deprived rats by up-regulating Cry expression.

Key words

Jiao-tai-wan PI3K/AKT signaling cryptochrome sleep deprivation inflammation insulin resistance 


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Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Wen-ya Huang
    • 1
  • Xin Zou
    • 1
  • Fu-er Lu
    • 1
  • Hao Su
    • 1
  • Chu Zhang
    • 1
  • Yan-lin Ren
    • 1
  • Ke Fang
    • 1
  • Li-jun Xu
    • 1
  • Kai-fu Wang
    • 1
  • Qing-jie Chen
    • 2
  • Hui Dong
    • 1
    Email author
  1. 1.Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Biochemistry and Molecular Biology, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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