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Current Medical Science

, Volume 38, Issue 2, pp 324–328 | Cite as

Measurement Uncertainty Assessment for Determination of Allura Red in Puffed Food by HPLC

  • Ying Chen
  • Yun Zhao
  • Hua-Ming Wang
  • Qing Wang
  • Xi Chen
  • Dong-qun Xu
  • Tao Li
  • Feng Yu
  • Xiao-yong Wang
  • Jia-fa LiuEmail author
Article
  • 24 Downloads

Summary

This study aimed to assess the measurement uncertainty of a new method for determination of allura redin food by high performance liquid chromatography (HPLC). The uncertainty of mathematical model of allura red is based on Europe for Analytical Chemistry(EURACHEM) guidelines. The sources and components of uncertainty were calculated, including recovery, working solution, sample mass, final volume, response of standard solution, response of sample solution. The expanded uncertainty was 0.0024 (k=2). Uncertainty of working solution was the most significant factor contributing to the total uncertainty, accounting for 86.2%. The uncertainty of volume accounted for the minimum at 0.025%. The developed method is simple and accurate, which can be used for the determination of allura redin puffed samples.

Key words

uncertainty assessment allura red high performance liquid chromatography 

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Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Ying Chen
    • 1
    • 4
  • Yun Zhao
    • 2
  • Hua-Ming Wang
    • 1
  • Qing Wang
    • 3
  • Xi Chen
    • 3
  • Dong-qun Xu
    • 3
  • Tao Li
    • 4
  • Feng Yu
    • 4
  • Xiao-yong Wang
    • 4
  • Jia-fa Liu
    • 1
    Email author
  1. 1.Institute of Health Inspection and TestingHubei Provincial Center for Disease Control and PreventionWuhanChina
  2. 2.Department of ObstericsMaternal and Child Health Hospital of Hubei ProvinceWuhanChina
  3. 3.Institute for Environmental Health and Related Product SafetyChinese Center for Disease Control and PreventionBeijingChina
  4. 4.Public Hospital Administration of Hubei ProvinceHealth And Family Planning Commission of Hubei ProvinceWuhanChina

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