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Current Medical Science

, Volume 38, Issue 1, pp 160–166 | Cite as

Effect of SrtA on Interspecies Adherence of Oral Bacteria

  • Ying Song (宋 颖)
  • Jin-zhi He (何金枝)
  • Ren-ke Wang (王人可)
  • Jing-zhi Ma (马净植)Email author
  • Ling Zou (邹 玲)Email author
Article

Abstract

This study aimed to study whether the Sortase A (srtA) gene helps mediate coaggregation and co-adherence between Streptococcus mutans (S. mutans) and other salivary bacteria. S. mutans UA159 and srtA-deficient mutant served as “bait” in classical co-aggregation assays and membrane-based co-adherence assays were used to examine interactions of S. mutans with Fusobacterium nucleatum (F. nucleatum), Streptococcus mitis (S. mitis), Streptococcus gordonii (S. gordonii), Streptococcus sanguis (S. sanguis), Actinomyces naeslundii (A. naeslundii) and Lactobacillus. Co-adherence assays were also performed using unfractionated saliva from healthy individuals. Co-adhering partners of S. mutans were sensitively detected using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Both UA159 and its srtA-deficient mutant bound to F. nucleatum but not to any of the other five salivary bacteria. The srtA-deficient mutant showed lower co-adherence with F.nucleatum. The two S. mutans strains also showed similar co-adherence profiles against unfractionated salivary bacteria, except that UA159 S. mutans but not the srtA-deficient bound to a Neisseria sp. under the same conditions. Deleting srtA reduces the ability of S. mutans to bind to F.nucleatum, but it does not appear to significantly affect the binding profile of S. mutans to bulk salivary bacteria.

Key words

co-aggregation co-adherence membrane-binding assay denaturing gradient gel electrophoresis Streptococcus mutans sortase A-deficient mutant strain 

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Copyright information

© Huazhong University of Science and Technology 2018

Authors and Affiliations

  • Ying Song (宋 颖)
    • 1
    • 2
  • Jin-zhi He (何金枝)
    • 1
    • 3
  • Ren-ke Wang (王人可)
    • 1
    • 3
  • Jing-zhi Ma (马净植)
    • 4
    Email author
  • Ling Zou (邹 玲)
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Oral DiseasesSichuan UniversityChengduChina
  2. 2.Department of Conservation Dentistry and EndodonticsStomatological Hospital of Chongqing Medical UniversityChongqingChina
  3. 3.Department of Conservation Dentistry and Endodontics, West China Hospital of StomatologySichuan UniversityChengduChina
  4. 4.Department of Stomatology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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