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Interferon-γ alters the immune-related miRNA expression of microvesicles derived from mesenchymal stem cells

  • Ai-qi Zhao (赵爱琪)
  • Hui Xie (谢 慧)
  • Sheng-yan Lin (林生彦)
  • Qian Lei (雷 倩)
  • Wen-xiang Ren (任文祥)
  • Fei Gao (高 飞)
  • Hao Guo (郭 豪)
  • An-yuan Guo (郭安源)
  • Zhi-chao Chen (陈智超)Email author
  • Hong-xiang Wang (王红祥)Email author
Article

Summary

Increasing studies have demonstrated that interferon gamma (IFN-γ), which serves as a critical inflammatory cytokine, is essential to induce the immunosuppressive effects of mesenchymal stem cells (MSCs). However, the mechanisms underlying the enhanced immunosuppressive effects of IFN-γ-stimulated MSCs (γMSCs) are not fully understood. MSC-derived microvesicles (MSC-MVs) have been viewed as potential pivotal mediators of the immunosuppressive effects of MSCs. Moreover, microRNAs (miRNAs) are important regulators of immunological processes and can be shuttled from cell to cell by MVs. The aim of our study was to analyze the the miRNA expression signature of MVs derived from γMSCs (γMSC-MVs), which may provide better understanding of the immunosuppressive property of their parent cells. Through miRNA microarray and bioinformatics analysis, we found 62 significantly differentially expressed miRNAs (DEMs) in γMSC-MVs compared with MSC-MVs. And the potential target genes and signaling pathways regulated by DEMs were predicted and analyzed. Interestingly, many DEMs and predicted signaling pathways had been demonstrated to be involved in immunoregulation. Furthermore, the network between immunoregulation-related pathways and relevant DEMs was constructed. Collectively, our research on the miRNA repertoires of γMSC-MVs not only provides new perspectives into the mechanisms underlying the enhanced immunosuppressive property of γMSCs, but also paves the way to clinical application of these potent organelles in the future.

Key words

mesenchymal stem cells interferon gamma microvesicles microRNAs 

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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Ai-qi Zhao (赵爱琪)
    • 1
  • Hui Xie (谢 慧)
    • 2
  • Sheng-yan Lin (林生彦)
    • 3
  • Qian Lei (雷 倩)
    • 1
  • Wen-xiang Ren (任文祥)
    • 1
  • Fei Gao (高 飞)
    • 1
  • Hao Guo (郭 豪)
    • 1
  • An-yuan Guo (郭安源)
    • 3
  • Zhi-chao Chen (陈智超)
    • 1
    Email author
  • Hong-xiang Wang (王红祥)
    • 4
    Email author
  1. 1.Institute of Hematology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Geriatrics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  3. 3.Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Bioinformatics and Systems Biology, Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and TechnologyHuazhong University of Science and TechnologyWuhanChina
  4. 4.Department of HematologyWuhan Central HospitalWuhanChina

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