Role of axl in preeclamptic EPCs functions

  • Ying Hu (胡 颖)
  • Xiao-ping Liu (刘小平)
  • Xiao-xia Liu (刘晓夏)
  • Yan-fang Zheng (郑艳芳)
  • Wei-fang Liu (刘维芳)
  • Ming-lian Luo (骆名恋)
  • Hui Gao (高 慧)
  • Ying Zhao (赵 茵)
  • Li Zou (邹 丽)
Article
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Summary

Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P<0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.

Key words

Axl endothelial progenitor cells preeclampsia 
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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ying Hu (胡 颖)
    • 1
  • Xiao-ping Liu (刘小平)
    • 1
  • Xiao-xia Liu (刘晓夏)
    • 1
  • Yan-fang Zheng (郑艳芳)
    • 1
  • Wei-fang Liu (刘维芳)
    • 1
  • Ming-lian Luo (骆名恋)
    • 1
  • Hui Gao (高 慧)
    • 1
  • Ying Zhao (赵 茵)
    • 1
  • Li Zou (邹 丽)
    • 1
  1. 1.Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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