Neamine inhibits growth of pancreatic cancer cells In Vitro and In Vivo

  • Ya-ping Liu (刘亚萍)
  • Yan-li Wu (吴艳丽)
  • Xiao-yan Zhang (章晓燕)
  • Guo-fu Hu (胡国富)
  • Yun-xia Wu (吴云霞)Email author


Neamine, a non-toxic derivative of neomycin, has recently been shown to have antitumor activities in various types of cancers. However, its effect on pancreatic cancer is still unknown. The study aimed to investigate its antitumor activity on pancreatic cancer and the underlying mechanisms. MTT assay was used to observe the effect of neamine on angiogenin (ANG)-induced AsPC-1 cell proliferation. Tissue microassay and immunofluorescence staining were used to detect the expression of ANG and its nuclear translocation, respectively. Tumor xenografts were established by subcutaneous inoculation of AsPC-1 pancreatic cancer cells into the right flanks of nude mice, and neamine was injected subcutaneously. Immunohistochemistry was done to observe the expression of ANG, CD31 and Ki-67 in tumor xenografts. It was found that neamine blocked the nuclear translocation of ANG effectively and inhibited ANG-induced AsPC-1 cell proliferation in a dose-dependent manner. Neamine had anti-tumor effects on AsPC-1 xenograft models. Consistently, neamine reduced the expression levels of ANG, Ki-67 and CD31 in tumor xenografts. It was concluded that neamine may be a promising agent for treatment of pancreatic cancer.

Key words

neamine angiogenin pancreatic cancer cell proliferation angiogenesis 


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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Ya-ping Liu (刘亚萍)
    • 1
  • Yan-li Wu (吴艳丽)
    • 1
  • Xiao-yan Zhang (章晓燕)
    • 1
  • Guo-fu Hu (胡国富)
    • 2
  • Yun-xia Wu (吴云霞)
    • 1
    Email author
  1. 1.School of Pharmacy, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Molecular Oncology Research InstituteTufts Medical CenterBostonUSA

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