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Total triterpenoids from Ganoderma Lucidum suppresses prostate cancer cell growth by inducing growth arrest and apoptosis

  • Tao Wang (王 涛)
  • Zi-ping Xie (谢子平)
  • Zhan-sen Huang (黄展森)
  • Hao Li (李 浩)
  • An-yang Wei (韦安阳)Email author
  • Jin-ming Di (狄金明)Email author
  • Heng-jun Xiao (肖恒军)Email author
  • Zhi-gang Zhang (张志刚)Email author
  • Liu-hong Cai (蔡柳洪)Email author
  • Xin Tao (陶 欣)Email author
  • Tao Qi (齐 涛)Email author
  • Di-ling Chen (陈地灵)Email author
  • Jun Chen (陈 俊)Email author
Article

Summary

In this study, one immortalized human normal prostatic epithelial cell line (BPH) and four human prostate cancer cell lines (LNCaP, 22Rv1, PC-3, and DU-145) were treated with Ganoderma Lucidum triterpenoids (GLT) at different doses and for different time periods. Cell viability, apoptosis, and cell cycle were analyzed using flow cytometry and chemical assays. Gene expression and binding to DNA were assessed using real-time PCR and Western blotting. It was found that GLT dose-dependently inhibited prostate cancer cell growth through induction of apoptosis and cell cycle arrest at G1 phase. GLT-induced apoptosis was due to activation of Caspases-9 and -3 and turning on the downstream apoptotic events. GLT-induced cell cycle arrest (mainly G1 arrest) was due to up-regulation of p21 expression at the early time and down-regulation of cyclin-dependent kinase 4 (CDK4) and E2F1 expression at the late time. These findings demonstrate that GLT suppresses prostate cancer cell growth by inducing growth arrest and apoptosis, which might suggest that GLT or Ganoderma Lucidum could be used as a potential therapeutic drug for prostate cancer.

Key words

Ganoderma Lucidum triterpenoids prostate cancer cell cycle arrest apoptosis 

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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  1. 1.Department of Urology, Medical Center for Overseas Patients, Nanfang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Department of UrologyLongjiang Hospital of Shunde District in Foshan CityGuangdongChina
  3. 3.Department of Andrology, Renmin HospitalHubei University of MedicineShiyanChina
  4. 4.Department of Infertility and Sexual Medicinethe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  5. 5.Department of Urologythe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou, GuangdongChina
  6. 6.Department of Pathologythe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  7. 7.Center for Reproductive Medicinethe Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  8. 8.State Key Laboratory of Applied MicrobiologySouth China, Guangdong Institute of MicrobiologyGuangzhouChina

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