Hepatoprotective activity of the ethanol extract of Sarcopyramis Nepalensis

  • Jieru Guo (郭洁茹)
  • Jinwen Zhang (张锦文)
  • Guangmin Yao (姚广民)
  • Mei Liao (廖 梅)
  • Huadong Chen (陈华栋)
  • Xixiong Yang (杨希雄)
  • Yonghui Zhang (张勇慧)
Article
  • 105 Downloads

Summary

The present study examined the protective effect of the ethanol extract of Sarcopyramis nepalensis (EESN) on agents-induced hepatotoxicity in mice and the possible mechanism. Acute liver injury was induced by administration of either CCl4 or D-GalN. The animals were divided into 5 groups in terms of different treatment: normal group, CCl4 or D-GalN group, silymarin or bifendate group, low dose EESN group (10 mg/kg) and high dose EESN group (30 mg/kg). Liver function was evaluated by detecting the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The oxidize stress markers were measured, including malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD). Liver tissues were histopathologically examined by hematoxylin-eosin (H&E) staining. The acute toxicity study revealed that there was no toxicity of EESN at the dose of 5 g/kg in mice. The levels of ALT and AST in serum, and the MDA level in live tissues were significantly increased and the activities of SOD and GSH substantially decreased in mice after CCl4 or D-GalN treatment. These biochemical and oxidize stress markers were profoundly improved after treatment with EESN at different doses, which was similar to the results of silymarin or bifendate treatment. The histophathological examination revealed the significant improvement in the pathological changes of the liver in EESN-treated mice as compared to those in CCl4 or D-GalN group. It was concluded that EESN possesses potential antioxidant and hepatoprotective properties and has therapeutic potential for liver diseases.

Key words

Sarcopyramis nepalensis hepatoprotective activity CCl4, D-GalN 

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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jieru Guo (郭洁茹)
    • 1
    • 2
  • Jinwen Zhang (张锦文)
    • 1
    • 3
  • Guangmin Yao (姚广民)
    • 1
    • 2
  • Mei Liao (廖 梅)
    • 1
    • 2
  • Huadong Chen (陈华栋)
    • 1
    • 2
  • Xixiong Yang (杨希雄)
    • 4
  • Yonghui Zhang (张勇慧)
    • 1
    • 3
  1. 1.School of Pharmacy, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Hubei Key Laboratory of Natural Medicinal Chemistry and Resource EvaluationWuhanChina
  3. 3.Department of Pharmacy, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  4. 4.College of Chemical Engineering and PharmacyJingchu University of TechnologyJingmenChina

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