Inhibitory effects of miR-146b-5p on cell migration and invasion of pancreatic cancer by targeting MMP16

  • Fan Lin (林 凡)
  • Xin Wang (王 欣)
  • Zhigang Jie (揭志刚)
  • Xiaoquan Hong (洪晓泉)
  • Xu Li (李 旭)
  • Min Wang (王 敏)
  • Yahong Yu (俞亚红)
Article

Summary

Previous studies have shown that miRNAs participate in a wide range of biological functions and play important roles in various human diseases including cancer. We found miR-146b-5p significantly dysregulated in human pancreatic cancer cells by qRT-PCR. To demonstrate its function and regulation mechanism, we overexpressed miR-146-5p by transfecting the mimics. Our data showed that miR-146b-5p overexpression significantly reduced the abilities of migration and invasion of MIA PaCa-2 pancreatic cancer cells. Furthermore, we found that matrix metalloproteinase 16 (MMP16) was a downstream target of miR-146b-5p by dual-luciferase reporter assay. Altogether, our findings suggest that miR-146b-5p may be involved in pancreatic cancer cell migration and invasion by targeting MMP16, and miR-146b-5p may be a potential therapeutic target for the pancreatic cancer.

Key words

miR-146b-5p pancreatic cancer matrix metalloproteinase migration invasion 

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Fan Lin (林 凡)
    • 1
  • Xin Wang (王 欣)
    • 1
  • Zhigang Jie (揭志刚)
    • 1
  • Xiaoquan Hong (洪晓泉)
    • 1
  • Xu Li (李 旭)
    • 1
  • Min Wang (王 敏)
    • 1
  • Yahong Yu (俞亚红)
    • 1
  1. 1.Department of Biliopancreatic Surgery, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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