Effects of maternal serum on permeability of glomerular endothelial cell membrane

  • Hui Du (杜 慧)
  • Haiyi Liu (刘海意)
  • Jun Zhao (赵 君)
  • Yuanyuan Wu (吴媛媛)
  • Xun Gong (龚 洵)
  • Qiong Zhou (周 琼)
  • jingjing Xu (徐晶晶)
  • Yuqi Li (李宇琪)
  • Xinwei Shi (石鑫玮)
  • Fuyuan Qiao (乔福元)
Article

DOI: 10.1007/s11596-011-0143-7

Cite this article as:
Du, H., Liu, H., Zhao, J. et al. J. Huazhong Univ. Sci. Technol. [Med. Sci.] (2011) 31: 17. doi:10.1007/s11596-011-0143-7

Summary

The mechanism of injury on the human glomerular endothelial cells (ciGENC) induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader. Morphologic change of ciGENC was observed under inverted phase contrast microscope. The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group (P<0.01). Permeability in preeclampsia groups was significantly increased as compared with control group (P<0.01). By contrast with severe preeclampsia group, the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly (P<0.05). Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups. It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function.

Key words

preeclampsia serum glomerular endothelial cells permeability sFlt-1 VEGF 

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • Hui Du (杜 慧)
    • 1
  • Haiyi Liu (刘海意)
    • 1
  • Jun Zhao (赵 君)
    • 1
  • Yuanyuan Wu (吴媛媛)
    • 1
  • Xun Gong (龚 洵)
    • 1
  • Qiong Zhou (周 琼)
    • 1
  • jingjing Xu (徐晶晶)
    • 1
  • Yuqi Li (李宇琪)
    • 1
  • Xinwei Shi (石鑫玮)
    • 1
  • Fuyuan Qiao (乔福元)
    • 1
  1. 1.Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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