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Effects of Ad-p27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice

  • Xianxiang Chen (陈先祥)
  • Weixing Wang (王卫星)
  • Linfei Zhang (张林菲)
  • Zhijun He (何志军)
  • Qinghe Cai (蔡庆和)
  • Xiaobo Liu (刘小波)
  • Caitao Cheng (程彩涛)
  • Liming Wu (吴黎明)
Article

Summary

In this study, the mechanism by which Ad-p27mt inhibits the growth, invasion and metastasis of transplanted liver tumor was studied by examining the effects of Ad-27mt gene transfer on the expression of Bax, Bcl-2, VEGF and MMP-9 in the transplanted liver tumors in nude mice. The model of transplanted hepatic tumor was established in nude mice. The mice were then divided into three groups, which were injected with PBS, Ad-LacZ and Ad-p27mt and the growth of the transplanted liver tumor was observed. The expressions of P27, Bax and Bcl-2 proteins were detected by Western blotting and the expressions of VEGF and MMP-9 were immunohistochemically determined. Our result showed that the tumor size, expressions of Bax, Bcl-2 proteins, VEGF and MMP-9 were all lower than those in PBS and Ad-LacZ groups and the differences were statistically significant (P<0.05). Our study suggested that Ad-p27mt could inhibit the growth, invasion and metastasis of hepatic cancer by lowering the expressions of VEGF and MMP-9.

Key words

Ad-p27mt hepatocelluar carcinoma transplanted tumors 

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Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Xianxiang Chen (陈先祥)
    • 1
    • 2
  • Weixing Wang (王卫星)
    • 1
  • Linfei Zhang (张林菲)
    • 2
  • Zhijun He (何志军)
    • 2
  • Qinghe Cai (蔡庆和)
    • 2
  • Xiaobo Liu (刘小波)
    • 2
  • Caitao Cheng (程彩涛)
    • 2
  • Liming Wu (吴黎明)
    • 2
  1. 1.Department of Hepatobiliary and Laparoscopic Surgery, Renmin HospitalWuhan UniversityWuhanChina
  2. 2.Department of Hepatobiliary and Pancreatic Surgery, Renmin HospitalHubei Medical UniversityShiyanChina

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