Aortic cell apoptosis in rat primary aldosteronism model

  • Yongji Yan (闫永吉)
  • Jinzhi Ouyang (欧阳金芝)
  • Chao Wang (王超)
  • Zhun Wu (吴 准)
  • Xin Ma (马 鑫)
  • Hongzhao Li (李宏召)
  • Hua Xu (徐 华)
  • Zheng Hu (胡 争)
  • Jun Li (李 俊)
  • Baojun Wang (王保军)
  • Taoping Shi (史涛坪)
  • Daojing Gong (龚道静)
  • Dong Ni (倪 栋)
  • Xu Zhang (张 旭)
Article
  • 70 Downloads

Summary

This study aimed to determine whether aldosterone could induce vascular cell apoptosis in vivo. Thirty-two male rats were randomly divided into 4 groups: vehicle (control), aldosterone, aldosterone plus eplerenone or hydralazine. They were then implanted with an osmotic mini-pump that infused either aldosterone or the vehicle. Systolic blood pressure (SBP) was measured weekly by the tail-cuff method. After 8 weeks, plasma aldosterone concentration (PAC) and renin activity (PRA) were determined by radioimmunoassay. Aortic apoptosis was examined by TUNEL assay. The levels of cytochrome c and caspase-3 were determined by Western blotting and the expression of Bax and Bcl-2 was detected by immnuohistochemistry and Western blotting. The results showed that as compared with control group, aldosterone-infused rats exhibited: (1) an increase in SBP; (2) significantly elevated PAC with depressed PRA; (3) elevated aortic vascular cell apoptosis accompanied with higher levels of cytochrome c and activated caspase-3; and (4) significantly up-regulated Bax protein with down-regulated Bcl-2. These effects of aldosterone were significantly inhibited after co-administration with eplerenone but not with hydralazine. It was concluded that aldosterone induced vascular cell apoptosis by its direct effect on the aorta via mineralocorticoid receptors and independently of blood pressure, which may contribute to aldosterone-mediated vascular injury.

Key words

aldosteronism apoptosis mineralocorticoid receptor aorta 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Yongji Yan (闫永吉)
    • 1
    • 2
  • Jinzhi Ouyang (欧阳金芝)
    • 3
  • Chao Wang (王超)
    • 4
  • Zhun Wu (吴 准)
    • 1
  • Xin Ma (马 鑫)
    • 2
  • Hongzhao Li (李宏召)
    • 2
  • Hua Xu (徐 华)
    • 1
  • Zheng Hu (胡 争)
    • 1
  • Jun Li (李 俊)
    • 1
  • Baojun Wang (王保军)
    • 2
  • Taoping Shi (史涛坪)
    • 2
  • Daojing Gong (龚道静)
    • 1
  • Dong Ni (倪 栋)
    • 1
  • Xu Zhang (张 旭)
    • 1
    • 2
  1. 1.Department of Urology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Urology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical CollegeBeijingChina
  3. 3.Department of Endocrinology, Chinese People’s Liberation Army General HospitalMilitary Postgraduate Medical CollegeBeijingChina
  4. 4.Department of UrologyJining First People’s HospitalJiningChina

Personalised recommendations