In vitro study on human trophoblast cells infected with HCMV

  • Juan Xiao (肖 娟)
  • Dandan Zhang (张丹丹)
  • Juanjuan Chen (陈娟娟)
  • Zongzhi Yin (尹宗智)
  • Tao Liu (刘 涛)
  • Jihui Ai (艾继辉)
  • Suhua Chen (陈素华)
Article

Summary

Human trophoblast cells were isolated and cultured in vitro in order to investigate possible pathogenesis of intrauterine infection caused by HCMV. Trophoblast cells were obtained by compound enzymes digestion and discontinuous percoll gradient. Cells and purity were identified by using immunocytochemistry assay with anti-CK7, Vim and β-hCG antibodies. HCMV AD169 strain replication in isolated trophoblast cells and cell apoptosis were detected at different time points post infection (p.i.). The results showed that highly purified trophoblast cells were obtained. Specific virus replication was increased dramatically at the 24th h p.i., and then increased slowly during 48 h and 72 h. Apoptosis rate of trophoblast cells infected with HCMV was (34.68±3.14)% at 24th h p.i., while that in control group was (15.32±2.34)% (P<0.05). It was suggested that highly purified trophoblast cells can be isolated by the simplified cell purification method. HCMV can infect human trophoblast cells, and be quickly replicated, resulting in the accelerated apoptosis of human trophoblast cells during early time.

Key words

cytomegalovirus trophoblast cells isolation and purification biological characteristics 

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Copyright information

© Huazhong University of Science and Technology and Springer Berlin Heidelberg 2010

Authors and Affiliations

  • Juan Xiao (肖 娟)
    • 1
  • Dandan Zhang (张丹丹)
    • 1
  • Juanjuan Chen (陈娟娟)
    • 1
  • Zongzhi Yin (尹宗智)
    • 1
  • Tao Liu (刘 涛)
    • 1
  • Jihui Ai (艾继辉)
    • 1
  • Suhua Chen (陈素华)
    • 1
  1. 1.Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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