Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model
- 95 Downloads
To examine the effect of transcatheter arterial embolization (TAE) of liver tumors on hypoxia-inducible factor-1α (HIF-1α) expression in the residual viable tumor, a total of 30 New Zealand White rabbits implanted with VX2 liver tumor were divided into 2 groups. TAE-treated group animals (n=15) were subjected to TAE with 150–250 μm polyvinyl alcohol particles. Control group animals (n=15) underwent sham embolization with distilled water. Six hours, 3 days or 7 days after TAE, the animals were sacrificed, and samples of tumor and adjacent normal liver tissue were harvested. Expression of HIF-1α protein was examined immunohistochemically. Real-time PCR was performed to examine the HIF-1α mRNA levels. Our results showed that HIF-1α protein was expressed in the VX2 tumors but not in the adjacent normal liver tissue. The HIF-1α-positive tumor cells were located predominantly at the periphery of necrotic tumor regions. The mean levels of HIF-1α protein were significantly higher in TAE-treated tumors than those in control tumors (P=0.002). Among the three sacrificing time points, the difference in increase in HIF-1α protein was significant between the two groups at the sacrificing time point of 6 h and 3 days after TAE (P=0.020, P=0.031, respectively), whereas no significant increase was noted 7 days after TAE (P=0.502). In contrast, although HIF-1α mRNA was expressed in TAE-treated and control VX2 tumors, there existed no significant difference in the HIF-1α mRNA level between the two groups (P=0.372). It is concluded that TAE of liver tumors increases the expression of HIF-1α at protein level in the residual viable tumor, which could be attributed to hypoxia generated by the procedure.
Key wordsembolization hypoxia-inducible factor-1 liver neoplasms
Unable to display preview. Download preview PDF.
- 9.Huang J, He X, Lin X, et al. Effect of preoperative transcatheter arterial chemoembolization on tumor cell activity in hepatocellular carcinoma. Chin Med J (Engl), 2000,113(5):446–448Google Scholar
- 21.Wang Y, Feng GS, Qian J, et al. Effect of hepatic arterial blockage on HIF-1α and VEGF expression of transplanted hepatoma in rats. J Clin Radiol (Chinese), 2006,25(8): 775–777Google Scholar
- 24.Su C, Zhang HZ, Li WH, et al. Culture in vitro and related biological characteristics of rabbit VX2 carcinoma cells. J Fourth Mil Med Univ (Chinese), 2006,27(9):844–847Google Scholar