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Expression of hypoxia-inducible factor-1α in liver tumors after transcatheter arterial embolization in an animal model

  • Bin Liang (梁 斌)
  • Chuansheng Zheng (郑传胜)
  • Gansheng Feng (冯敢生)
  • Yong Wang (王 勇)
  • Hui Zhao (赵 辉)
  • Huimin Liang (梁惠民)
  • Enhua Xiao (肖恩华)
Article

Summary

To examine the effect of transcatheter arterial embolization (TAE) of liver tumors on hypoxia-inducible factor-1α (HIF-1α) expression in the residual viable tumor, a total of 30 New Zealand White rabbits implanted with VX2 liver tumor were divided into 2 groups. TAE-treated group animals (n=15) were subjected to TAE with 150–250 μm polyvinyl alcohol particles. Control group animals (n=15) underwent sham embolization with distilled water. Six hours, 3 days or 7 days after TAE, the animals were sacrificed, and samples of tumor and adjacent normal liver tissue were harvested. Expression of HIF-1α protein was examined immunohistochemically. Real-time PCR was performed to examine the HIF-1α mRNA levels. Our results showed that HIF-1α protein was expressed in the VX2 tumors but not in the adjacent normal liver tissue. The HIF-1α-positive tumor cells were located predominantly at the periphery of necrotic tumor regions. The mean levels of HIF-1α protein were significantly higher in TAE-treated tumors than those in control tumors (P=0.002). Among the three sacrificing time points, the difference in increase in HIF-1α protein was significant between the two groups at the sacrificing time point of 6 h and 3 days after TAE (P=0.020, P=0.031, respectively), whereas no significant increase was noted 7 days after TAE (P=0.502). In contrast, although HIF-1α mRNA was expressed in TAE-treated and control VX2 tumors, there existed no significant difference in the HIF-1α mRNA level between the two groups (P=0.372). It is concluded that TAE of liver tumors increases the expression of HIF-1α at protein level in the residual viable tumor, which could be attributed to hypoxia generated by the procedure.

Key words

embolization hypoxia-inducible factor-1 liver neoplasms 

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Copyright information

© Huazhong University of Science and Technology and Springer Berlin Heidelberg 2009

Authors and Affiliations

  • Bin Liang (梁 斌)
    • 1
  • Chuansheng Zheng (郑传胜)
    • 1
  • Gansheng Feng (冯敢生)
    • 1
  • Yong Wang (王 勇)
    • 1
  • Hui Zhao (赵 辉)
    • 1
  • Huimin Liang (梁惠民)
    • 1
  • Enhua Xiao (肖恩华)
    • 2
  1. 1.Department of Radiology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Radiology, the Second Xiangya Hospital, Xiangya School of MedicineCentral South UniversityChangshaChina

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