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Skeletal muscle-derived stem cells exhibit cardiocyte competences

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Summary

Adult stem cells from skeletal muscle cells were induced to differentiate into cardiocytes to see if stem cells from another different but histologically-comparable tissues can differentiate to the target cells. Skeletal muscles-derived stem cells (MDSCs) were isolated from adult skeleton muscle tissues by differential adhesion, and immunocytochemically identified by using Sca-1. In order to induce the proliferation but not differentiation of MDSCs, the cells were cultured in Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) supplemented with 1:50 B27, 20 ng/mL basic fibroblast growth factor (bFGF), 20 ng/mL epidermal growth factor (EGF) in a suspension for 6 days. Then these stem cells were treated with 5 μmol/L 5-azacytidine for 24 h in an adherence culture. The characteristics of induced cells were examined by immunocytochemistry, quantitative real time RT-PCR and morphological observation of cell phenotype. Our results showed that the appearance of some cells gradually changed from spindle-shape into polygonal or short-column-shape. Some of these post-treated cells could contract spontaneously and rhythmically. The expression of GATA-4 and cTnT was increased 1 and 2 week(s) after the treatment. And about 16.6% of post-treated cells were cTnT-positive. Therefore, we are led to conclude that skeletal muscle-derived stem cells could differentiate into cardiocyte-like cells, which exhibited some characteristics of cardiocytes.

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Authors and Affiliations

  1. Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jin Li  (李 进), Guangxiang Hong  (洪光祥), Jianghai Chen  (陈江海), Hao Kang  (康 皓) & Zhenbin Chen  (陈振兵)

  2. Department of Orthopedics, Yingshan County People’s Hospital, Huanggang, 438700, China

    Desheng Fu  (付德生)

Authors
  1. Jin Li  (李 进)
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  2. Desheng Fu  (付德生)
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  3. Guangxiang Hong  (洪光祥)
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  4. Jianghai Chen  (陈江海)
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  5. Hao Kang  (康 皓)
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  6. Zhenbin Chen  (陈振兵)
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Corresponding author

Correspondence to Jin Li  (李 进).

Additional information

This study was supported by a grant from the National Natural Sciences Foundation of China (No. 30872627) and Hubei Provincial Natural Sciences Foundation (No. 2007ABA133).

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Li, J., Fu, D., Hong, G. et al. Skeletal muscle-derived stem cells exhibit cardiocyte competences. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 29, 741–744 (2009). https://doi.org/10.1007/s11596-009-0614-2

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  • DOI: https://doi.org/10.1007/s11596-009-0614-2

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