Advertisement

Apoptosis of HeLa cells induced by cisplatin and its mechanism

  • Youqing Liu (柳友清)
  • Hui Xing (邢辉)
  • Xiaobing Han (韩晓兵)
  • Xiaoyan Shi (石小燕)
  • Fengqi Liang (梁缝奇)
  • Gang Cheng (陈刚)
  • Yunping Lu (卢运萍)
  • Ding Ma (马丁)
Article

Summary

To study the apoptosis induced by cisplatin in cervical cancer cell line HeLa and its mechanism, cell growth inhibition of cisplatin on HeLa cells was analyzed by MTT assay. Cell apoptosis was examined by cytometry and Hoechst33258 staining after treatment with cisplatin. The effects of cisplatin on transcription of E6 were analyzed by RT-PCR. The protein expressions of E6, p53, p21, Bax and Bcl-2 were studied by Western blotting. Cisplatin inhibited proliferation in a time-and dose-dependant manner. Cytometically, sub-G1 peak showed higher apoptosis rates in the experimental group than those in the control. Hoechst33258staining exhibited apoptosis induced by cisplatin. RT-PCR revealed that cisplatin decreased transcription of E6. Western blotting showed that cisplatin decreased protein expression of E6 and increased protein expression of p53, p21 and Bax. It had no effect on protein expression of Bcl-2. It is concluded that cisplatin can induce apoptosis in HeLa cells by suppressing HPV E6 and thereby restoring the function of p53.

Key words

cell apoptosis cisplatin flow cytometry HPV E6 Bax Bcl-2 cervix cancer 

References

  1. 1.
    Walboomers J M, Jacobs M V, Manos M M et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol, 1999,189(1):12PubMedCrossRefGoogle Scholar
  2. 2.
    Cohen S M, Lippard S J. Cisplatin: from DNA damage to cancer chemotherapy. Prog Nucleic Acid Res Mol Biol, 2001,67:93PubMedCrossRefGoogle Scholar
  3. 3.
    Fulda S, Debatin K M. Apoptosis signaling in tumor therapy. Ann N Y Acad Sci, 2004,1028:150PubMedCrossRefGoogle Scholar
  4. 4.
    Muggia F M. Recent updates in the clinical use of platinum compounds for the treatment of gynecologic cancers. Semin Oncol, 2004, 31(6):17.PubMedCrossRefGoogle Scholar
  5. 5.
    Geng Y J. Molecular signal transduction in vascular cell apoptosis. Cell Res, 2001,11(4):253PubMedCrossRefGoogle Scholar
  6. 6.
    Hietanen S, Lain S, Krausz E et al. Activation of p53 in cervical carcinoma cells by small molecules. Pro Natl Acad Sci USA, 2000,18, 97(15):8501CrossRefGoogle Scholar
  7. 7.
    Chou R H, Huang H. Restoration of p53 tumor suppressor pathway in human cervical carcinoma cells by sodium arsenite. Biochem Biophys Res Commun, 2002,293(1):298PubMedCrossRefGoogle Scholar

Copyright information

© Huazhong University of Science and Technology and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Youqing Liu (柳友清)
    • 1
  • Hui Xing (邢辉)
    • 1
  • Xiaobing Han (韩晓兵)
    • 1
  • Xiaoyan Shi (石小燕)
    • 1
  • Fengqi Liang (梁缝奇)
    • 1
  • Gang Cheng (陈刚)
    • 1
  • Yunping Lu (卢运萍)
    • 1
  • Ding Ma (马丁)
    • 1
  1. 1.Department of Obstetrics and GynecologyTongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyWuhanChina

Personalised recommendations