Cloning, eukaryotic expression of human ISG20 and preliminary study on the effect of its anti-HBV

  • Youhua Hao (郝友华)
  • Dongliang Yang (杨东亮)


Human ISG20 gene was cloned and the effect of its anti-HBV was primarily studied. The ISG20 gene was amplified from HeLa cells by RT-PCR and recombinant vector expressing ISG20 was constructed by genetic engineering. The overexpression of ISG20 in HepG2 cells was detected by Western blot and the levels of secretion of HBs antigen and HBe antigen tested by ELISA. The results showed that: (1) Sequence of ISG20 cloned was consistent to that published in Genebank; (2) Recombinant vector expressing ISG20 could be expressed in HepG2 cells by transfection; (3) The overexpression of ISG20 protein could reduce the levels of the secretion of HBs antigen and HBe antigen in transfected HepG2 cells. It was suggested that the overexpression of recombinant ISG20 in culture cells could reduce the synthesis of HBV proteins.

Key words

human ISG20 effect of anti-HBV 


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  1. 1.
    Lee W M. Hepatitis B virus infection. N Engl J Med, 1997,337:1733–1745PubMedCrossRefGoogle Scholar
  2. 2.
    Dienstag J L, Perrillo R P, Schiff ER. A preliminary trial of lamivudine for chronic hepatitis B infection. N Engl J Med, 1995,333:1657–1661PubMedCrossRefGoogle Scholar
  3. 3.
    Platanias L C. Mechanisms of type-I-and type-II-interferon-mediated signaling. Nat Rev Immunol, 2005,5:375–386PubMedCrossRefGoogle Scholar
  4. 4.
    Espert L, Degols G, Gongora C et al. ISG20, a new interferon-induced RNase specific for single-stranded RNA, defines an alternative antiviral pathway against RNA genomic viruses. JBC, 2003,278:16 151–16 158CrossRefGoogle Scholar
  5. 5.
    Espert L, Degols G, LIN Y L et al. Interferon-induced exonuclease ISG20 exhibits an antiviral activity against human immunodeficiency virus type 1. J Gen Virol, 2005, 86:2221–2229PubMedCrossRefGoogle Scholar
  6. 6.
    Wieland S F, Vega RG, Muller R et al. Searching for interferon-induced genes that inhibit hepatitis B virus replication in transgenic mouse hepatocytes. J Virol, 2003,77:1227–1236PubMedCrossRefGoogle Scholar
  7. 7.
    Gongora C, David G, Pintard L et al. Molecular cloning of a new interferon-induced PML nuclear body-associated protein. J Biol Chem, 1997,272:19 457–19 463CrossRefGoogle Scholar
  8. 8.
    Wieland S, Thimme R, Purcell R et al. Genomic analysis of the host response to hepatitis B virus infection. PNAS, 2004,101:6669–6674PubMedCrossRefGoogle Scholar

Copyright information

© Huazhong University of Science and Technology 2008

Authors and Affiliations

  • Youhua Hao (郝友华)
    • 1
  • Dongliang Yang (杨东亮)
    • 1
  1. 1.Division of Clinical Immunology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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