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The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells

  • Lin Nengxing  (林能兴)
  • Huang Changzheng  (黄长征)
  • Tian Jin  (田进)
  • Tao Juan  (陶娟)
  • Zhang Jin  (张进)
  • Yang Lingyun  (杨凌云)
  • Li Yan  (李延)
  • Liu Yeqiang  (刘业强)
  • Chen Siyuan  (陈思远)
  • Shen Guanxin  (沈关心)
  • Li Jiawen  (李家文)
  • Wang Chunsen  (王椿森)
  • Tu Yating  (涂亚庭)
Article

Summary

In order to investigate the expression of endothelin receptor B (ETR-B) in human malignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.

Key words

melanoma endothelin receptor B endothelin 3 A375 SK-mel-1 

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References

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Copyright information

© Huazhong University of Science and Technology 2007

Authors and Affiliations

  • Lin Nengxing  (林能兴)
    • 1
  • Huang Changzheng  (黄长征)
    • 1
  • Tian Jin  (田进)
    • 1
  • Tao Juan  (陶娟)
    • 1
  • Zhang Jin  (张进)
    • 1
  • Yang Lingyun  (杨凌云)
    • 1
  • Li Yan  (李延)
    • 1
  • Liu Yeqiang  (刘业强)
    • 1
  • Chen Siyuan  (陈思远)
    • 1
  • Shen Guanxin  (沈关心)
    • 2
  • Li Jiawen  (李家文)
    • 1
  • Wang Chunsen  (王椿森)
    • 1
  • Tu Yating  (涂亚庭)
    • 1
  1. 1.Department of Dermatology, Union Hospital Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Faculty of Immunology, School of Basic Medical Sciences, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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