Modulatory effects of EPA and DHA on proliferation and apoptosis of pancreatic cancer cells
- 134 Downloads
- 13 Citations
Summary
In order to investigate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the proliferation, apoptosis of pancreatic cancer cell line SW1990 cells and the expression of cyclin E mRNA, the SW1990 cells were treated with different concentrations of EPA or DHA (20, 40, 60 μg/mL) for 0, 12, 24, 36 and 48 h respectively. By using MTT method, the inhibitory effects of EPA or DHA on the cell growth were assayed. Real time PCR was used to detect the expression changes of cyclin E mRNA after the SW1990 cells were treated with 40 μg/mL EPA or DHA for different time. Flow cytometry was used to test the changes of apoptostic rate in the SW1990 cells treated with different concentrations of EPA or DHA for 24 h. The results showed that EPA and DHA could inhibit the growth of SW1990 cells in a time-and concentration-dependent manner (P<0.01). EPA or DHA could also significantly inhibit the expression of cyclin E mRNA in a time-dependent manner (P<0.05). EPA or DHA could induce the apoptosis of SW1990 cells in a concentration-dependent manner (P<0.01). It was concluded that ω-3 fatty acid could inhibit the proliferation of pancreatic cancer cell line SW1990 cells and promote their apoptosis. The down-regulation of the cyclin E expression by ω-3 fatty acid might be one of the mechanisms for its anti-tumor effect on pancreatic cancer.
Key words
ω-3 fatty acid cyclin E apoptosis malignant tumorPreview
Unable to display preview. Download preview PDF.
References
- 1.Fernandez E, Gallus S, La Vecchia C et al. Nutrition and cancer risk: an overview. J Br Menopause Soc, 2006,12(4):139–142PubMedCrossRefGoogle Scholar
- 2.Tanaka H, Kageyama K, Kimura M et al. Promotive effects of hyperthermia on the inhibition of DNA synthesis in ehrlich ascites tumor cells by eicosapentaenoic and docosahexaenoic acids. Exp Oncol, 2006,28(3):203–208PubMedGoogle Scholar
- 3.Tsekos E, Reuter C, Stehle P et al. Perioperative administration of parenteral fish oil supplements in a routine clinical setting improves patient outcome after major abdominal surgery. Clin Nutr, 2004,23(3):325–330PubMedCrossRefGoogle Scholar
- 4.Ding W Q, Lind S E. Phospholipid hydroperoxide glutathione peroxidase plays a role in protecting cancer cells from docosahexaenoic acid-induced cytotoxicity. Mol Cancer Ther, 2007,6(4):1467–1474PubMedCrossRefGoogle Scholar
- 5.Aleem E, Kiyokawa H, Kaldis P et al. Cdc2-cyclin E complexes regulate the G1/S phase transition. Nat Cell Biol, 2005,7(8):831–836PubMedCrossRefGoogle Scholar
- 6.Lundberg A S, Weiberg R A. Functional inactivation of the retinoblastoma protein requires sequential modification by at least two distinct cyclin-cdk complexes. Mol Cell Biol, 1998, 18(11):753–762PubMedGoogle Scholar
- 7.Rosen D G, Yang G, Deavers M T et al. Cyclin E expression is correlated with tumor progression and predicts a poor prognosis in patients with ovarian carcinoma. Cancer, 2006, 106(9):1925–1932PubMedCrossRefGoogle Scholar
- 8.Skalicky D A, Kench J G, Segara D et al. Cyclin E expression and outcome in pancreatic ductal adenocarcinoma. Cancer Epidemiol Biomarkers Prev, 2006,15(10): 1941–1947PubMedCrossRefGoogle Scholar
- 9.Hardman W E. (n-3) fatty acids and cancer therapy. J Nutr, 2004,134(12 Suppl):3427S–3430SPubMedGoogle Scholar
- 10.Danbara N, Yuri T, Tsujita-Kyutoku M et al. Conjugated docosahexaenoic acid is a potent inducer of cell cycle arrest and apoptosis and inhibits growth of colo 201 human colon cancer cells. Nutr Cancer, 2004,50(1):71–79PubMedCrossRefGoogle Scholar