Influence of Lactobacillus E1 on the storage stability in emulsion immobilization

  • Xin Sun (孙欣)
  • Xiguang Chen (陈西广)
  • Chengsheng Liu
  • Dongsu Cha
  • Hongni Peng
Article

Abstract

A coacervation method with double emulsion strategy (w/o/w) was used to prepare immobilized Lactobacillus E1. Diatomite was chosen as the carrier for bacteria. Sodium alginate, dextrin and gelatin were used as protective solutes for the preservation of Lactobacillus E1 and their effects on the storage viability during storage were discussed. The influence of storage temperature on the storage viability was also examined. The results show that high bacteria viable count over 109 cfu/g for an extended shelf life of 37 d can be achieved with 2% sodium alginate, 5% dextrin and 4% gelatin as protective solutes, at 10°C of the storage temperature. This immobilized Lactobacillus E1 has potential use as functional food ingredient for both human dairy food and animal feedstuff.

Key words

lactobacillus microencapsulation storage viability protective solute shelf life 

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References

  1. [1]
    A C Huggett, B Schliter. Research Needs for Establishing the Safety of Functional Foods[J]. Nutr. Rev., 1996, 54: 143–148CrossRefGoogle Scholar
  2. [2]
    F Guarner, G J Schaafsma. Probiotics[J]. Int. J. Food Microbiol., 1998, 39: 237–238PubMedCrossRefGoogle Scholar
  3. [3]
    R Fuller. Probiotics in Man and Animals[J]. J. Appl. Bacteriol., 1989, 66: 365–378PubMedGoogle Scholar
  4. [4]
    C Shortt. The Probiotic Century: Historical and Current Perspectives[J]. Trends Food Sci. Technol., 1999, 10: 411–417CrossRefGoogle Scholar
  5. [5]
    A M P Gomes, X F Malcata. Bifidobacterium ssp. and Lactobacillus Acidophilus: Biological, Biochemical, Technological and Therapeutical Properties Relevant for Use as Probiotics[J]. Trends in Food Sci. Technol., 1999, 10: 139–157CrossRefGoogle Scholar
  6. [6]
    N Ishibashi, S Shimamura. Bifidobacteria: Research and Development in Japan[J]. Food Technol., 1993, 46: 126–135Google Scholar
  7. [7]
    A Talwalkar, K Kailasapathy. A Review of Oxygen Toxicity in Probiotic Yogurts: Influence on the Survival of Probiotic Bacteria and Protective Techniques[J]. Compr. Rev. Food Sci. Food Saf., 2004, 3: 117–124CrossRefGoogle Scholar
  8. [8]
    G A Birollo, J A Reinheimer, C G Vinderola. Viability of Lactic Acid Microflora in Different Types of Yoghurt[J]. Food Res. Int., 2000, 33: 799–805CrossRefGoogle Scholar
  9. [9]
    K Kailasapathy, S Rybka. Acidophilus and Bifidobacterium spp.-their Theraputic Potential and Survival in Yoghurt[J]. Aust. J. Dairy Technol., 1997, 52: 28–34Google Scholar
  10. [10]
    R R Ravula, N P Shah. Viability of Probiotic Bacteria in Fermented Dairy Deserts[J]. Food Aust., 1998, 50: 136–139Google Scholar
  11. [11]
    C Stanton, G Gardiner, H Meehan, et al. Market Potential for Probiotics[J]. Am. J. Clin. Nutr., 2001, 73: 4 765–4 835Google Scholar
  12. [12]
    I N Haynes, M J Playne. Survival of Probiotic Cultures in Low Fat Ice-cream[J]. Aust. J. Dairy Technol., 2002, 57: 10–14Google Scholar
  13. [13]
    K Hanny, M Lorna. Process for Producing Extended Shelf-life Ready-to-use Milk Compositions Containing Probiotics[P]. US, A23C 9/123, WO 02/102168 A1. 27. 12. 2002Google Scholar
  14. [14]
    R S Porubcan, R L Sellars. Lactic Starter Culture Concentrates[M]. In: Peppler, H J, Perlman, D. Microbial Technology. New York: Academic Press, 1979(1): 59–92Google Scholar
  15. [15]
    K Adhikari, A Mustapha, I U Grün. Survival and Metabolic Activity of Microencapsulated Bifidobacterium Longum in Stirred Yogurt[J]. J. Food Sci., 2003, 68: 275–280CrossRefGoogle Scholar
  16. [16]
    G Godward, K Kailasapathy. Viability and Survival of Free, Encapsulated and Co-encapsulated Probiotic Bacteria in Ice Cream[J]. Milchwissenschaft, 2003, 58: 161–164Google Scholar
  17. [17]
    C Amiet-Charpentier, J P Benoit, P Gadille, et al. Preparation of Rhizobacteria-containing Polymer Microparticles Using a Complex coacervation Method[J]. Colloids Surf., A: Physicochem. Eng. Aspects., 1998, 144: 179–190CrossRefGoogle Scholar
  18. [18]
    V A E King, H J Lin, C F Liu. Accelerated Storage Testing of Freeze-dried and Controlled Low-temperature Vacuum Dehydrated Lactobacillus Acidophilus[J]. J. Gen. Microbiol., 1998, 44: 161–165CrossRefGoogle Scholar
  19. [19]
    M Achour, N Mtimet, C Cornelius, et al. Application of the Accelerated Shelf Life Testing Method (ASLT) to Study the Survival Rates of Freeze-dried Lactococcus Starter Cultures[J]. J. Chem. Technol. Biotechnol., 2001, 76: 624–628CrossRefGoogle Scholar
  20. [20]
    P Myll.arinen, P Forssell, A von Wright, et al. Starch Capsules Containing Microorganisms and/or Polypeptides or Proteins and a Process for Producing them[P]. FI, A61K 9/52, WO 99/52511 A1. 21.10.99Google Scholar
  21. [21]
    R S Porubcan, M N Victoria. Formulations to Increase in Vivo Survival of Probiotic Bacteria and Extend their Shelf-life[P]. US, A61K 39/00, US2004/0175389 A1. 9. 9. 2004Google Scholar

Copyright information

© Wuhan University of Technology and Springer-Verlag GmbH 2009

Authors and Affiliations

  • Xin Sun (孙欣)
    • 1
  • Xiguang Chen (陈西广)
    • 1
  • Chengsheng Liu
    • 1
  • Dongsu Cha
    • 2
  • Hongni Peng
    • 1
  1. 1.College of Life ScienceOcean University of ChinaQingdaoChina
  2. 2.Graduate School of BiotechnologyKorea UniversitySeoulKorea

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