, Volume 25, Issue 2, pp 797–807 | Cite as

Preparation of cobalt-poly (naphthylamine)/sodium dodecylsulfate-modified carbon paste electrode as a sensitive sensor for l-cysteine

  • Banafsheh NorouziEmail author
  • Aria Gorji
Original Paper


A novel modified electrode for determination of l-cysteine (l-CySH) was described. The electrode comprises a cobalt-poly (naphthylamine)/sodium dodecyl sulfate (Co-PNA/SDS) as a modifier in the carbon paste matrix. This modified electrode has been produced by electropolymerization of naphthylamine in the presence of SDS and then incorporating of Co(II) ions. The electrochemical studies of this modified electrode (Co-PNA/SDS/MCPE) were done by using cyclic voltammetry. The experimental results exhibited the stable redox behavior of the Co(III)/Co(II) couple immobilized at the polymeric electrode, and this modified electrode is very sensitive to l-CySH. The current response increased linearly with l-CySH concentration over the range of 1–20 μM and 20–100 μM. The detection limit of the method was 0.8 μM (3δ). The prominent features of this sensor can be referred to simple preparation, low cost, fast response, good stability and selectivity, wide linear range, low detection limit, and high reproducibility.


Cobalt Naphthylamine SDS Polymer l-Cysteine 


  1. 1.
    Hammerich O, Ulstrup J (2008) Bioinorganic electrochemistry. Springer, NetherlandsCrossRefGoogle Scholar
  2. 2.
    Kulys J, Drungiliene A (1991) Chemically modified electrodes for the determination of sulfhydryl compounds. Anal Chim Acta 243:287–292CrossRefGoogle Scholar
  3. 3.
    Allison LA, Shoup RE (1983) Dual electrode liquid chromatography detector for thiols and disulfides. Anal Chem 55:8–12CrossRefGoogle Scholar
  4. 4.
    Palermo C, Joyce JA (2008) Cysteine cathepsin proteases as pharmacological targets in cancer. J Pharmacol Sci 29:22–28Google Scholar
  5. 5.
    Wring SA, Hart JP, Birch BJ (1989) Development of an improved carbon electrode chemically modified with cobalt phthalocyanine as a re-usable sensor for glutathione. Analyst 117:1563–1570CrossRefGoogle Scholar
  6. 6.
    Maleki N, Safavi A, Sedaghati F, Tajabadi F (2007) Efficient electrocatalysis of L-cysteine oxidation at carbon ionic liquid electrode. Anal Biochem 369:149–153CrossRefGoogle Scholar
  7. 7.
    Shahrokhian S (2001) Lead phthalocyanine as a selective carrier for preparation of a cysteine selective electrode. Anal Chem 73:5972–5978CrossRefGoogle Scholar
  8. 8.
    Weia X, Qia L, Tanb J, Liuc R, Wanga F (2010) A colorimetric sensor for determination of cysteine by carboxymethyl cellulose-functionalized gold nanoparticles. Anal Chim Acta 671:80–84CrossRefGoogle Scholar
  9. 9.
    Chen Z, Luo S, Liu C, Cai Q (2009) Simple and sensitive colorimetric detection of cysteine based on ss_DNA stabilized gold nanoparticles. Anal Bioanal Chem 395:489–494CrossRefGoogle Scholar
  10. 10.
    Nie LH, Ma HM, Sun M, Li XH, Su MH, Liang SC (2003) Direct chemiluminescence determination of cysteine in human serum using quinine–Ce(IV) system. Talanta 59:959–964CrossRefGoogle Scholar
  11. 11.
    Wang H, Wang WS, Zhang HS (2001) Spectrofluorimetic determination of cysteine based on the fluorescence inhibition of Cd(II)–8-hydroxyquinoline-5-sulphonic acid complex by cysteine. Talanta 53:1015–1019CrossRefGoogle Scholar
  12. 12.
    Ku’smierek K, Glowacki R, Bald E (2006) Analysis of urine for cysteine, cysteinylglycine, and homocysteine by high-performance liquid chromatography. Anal Bioanal Chem 385:855–860CrossRefGoogle Scholar
  13. 13.
    Nolin TD, McMenamin ME, Himmelfarb J (2007) Simultaneous determination of total homocysteine, cysteine, cysteinylglycine, and glutathione in human plasma by high-performance liquid chromatography: application to studies of oxidative stress. J Chromatogr B 852:554–561CrossRefGoogle Scholar
  14. 14.
    Possari R, Carvalhal RF, Mendes RK, Kubota LT (2006) Electrochemical detection of cysteine in a flow system based on reductive desorption of thiols from gold. Anal Chim Acta 575:172–179CrossRefGoogle Scholar
  15. 15.
    Rezaei B, Mokhtari A (2007) A simple and rapid flow injection chemiluminescence determination of cysteine with Ru(phen)3 2+–Ce(IV) system. Spectrochim Acta Part A 66:359–363CrossRefGoogle Scholar
  16. 16.
    Majidi MR, Asadpour-Zeynali K, Hafezi B (2010) Sensing l-cysteine in urine using a pencil graphite electrode modified with a copper hexacyanoferrate nanostructure. Microchim Acta 169:283–288CrossRefGoogle Scholar
  17. 17.
    Abbaspour A, Ghaffarinejad A (2008) Electrocatalytic oxidation of l-cysteine with a stable copper–cobalt hexacyanoferrate electrochemically modified carbon paste electrode. Electrochim Acta 53:6643–6650CrossRefGoogle Scholar
  18. 18.
    Sattarahmady N, Heli H (2011) An electrocatalytic transducer for l-cysteine detection based on cobalt hexacyanoferrate nanoparticles with a core-shell structure. Anal Biochem 409:74–80CrossRefGoogle Scholar
  19. 19.
    Tang X, Liu Y, Hou H, You T (2010) Electrochemical determination of l-tryptophan, l-tyrosine and l-cysteine using electrospun carbon nanofibers modified electrode. Talanta 80:2182–2186CrossRefGoogle Scholar
  20. 20.
    Tabeshnia M, Rashvandavei M, Amini R, Pashaee F (2010) Electrocatalytic oxidation of some amino acids on a cobalt hydroxide nanoparticles modified glassy carbon electrode. J Electroanal Chem 647:181–186CrossRefGoogle Scholar
  21. 21.
    Nezamzadeh-Ejhieh A, Hashemi HS (2012) Voltammetric determination of cysteine using carbon paste electrode modified with Co(II)-Y zeolite. Talanta 88:201–208CrossRefGoogle Scholar
  22. 22.
    Spataru N, Sarada BV, Popa E, Tryk DA, Fujishima A (2001) Voltammetric determination of l-cysteine at conductive diamond electrodes. Anal Chem 73:514–519CrossRefGoogle Scholar
  23. 23.
    Nekrassova O, Lawrence NS, Compton RG (2004) Selective electroanalytical assay for cysteine at a boron doped diamond electrode. Electroanalysis 16:1285–1291CrossRefGoogle Scholar
  24. 24.
    Nekrassova O, Lawrence NS, Compton RG (2003) Analytical determination of homocysteine. Talanta 60:1085–1095CrossRefGoogle Scholar
  25. 25.
    Hutchins MG, Wright PJ, Grebenik PD (1987) Comparison of different forms of black cobalt selective solar absorber surfaces. Sol Energy Mater 16:113–131CrossRefGoogle Scholar
  26. 26.
    Barrera E, Gonzales I, Viveros T (1988) A new cobalt oxide electrodeposit bath for solar absorbers. Sol Energy Mater Sol Cells 51:69–82CrossRefGoogle Scholar
  27. 27.
    Yoshino T, Baba N (1995) Characterization and properties of electrochromic cobalt oxide thin film prepared by electrodeposition. Sol Energy Mater Sol Cells 39:391–397CrossRefGoogle Scholar
  28. 28.
    Monk PMS, Ayub S (1997) Solid-state properties of thin film electrochromic cobalt–nickel oxide. Solid State Ionics 99:115–124CrossRefGoogle Scholar
  29. 29.
    Ueda Y, Kikuchi N, Ikeda S, Houga T (1999) Magnetoresistance and compositional modulation near the layer boundary of co/cu multilayers produced by pulse electrodeposition. J Magn Mater 198:40–742Google Scholar
  30. 30.
    Behl WK, Toni JE (1971) Anodic oxidation of cobalt in potassium hydroxide electrolyte. J Electroanal Chem 31:63–75CrossRefGoogle Scholar
  31. 31.
    Burke LD, Lyons ME, Murphy OJ (1982) Formation of hydrous oxide films on cobalt under potential cycling conditions. J Electroanal Chem 132:247–261CrossRefGoogle Scholar
  32. 32.
    Cataldi TRI, Guerrieri A, Casella IG, Desimoni E (1995) Study of a cobalt-based surface modified glassy carbon electrode: electrocatalytic oxidation of sugars and alditols. Electroanalysis 7:305–311CrossRefGoogle Scholar
  33. 33.
    Casella IG, Guascito MR (1999) Electrochemical preparation of a composite gold–cobalt electrode and its electrocatalytic activity in alkaline medium. Electrochim Acta 45:1113–1120CrossRefGoogle Scholar
  34. 34.
    Norouzi B, Sarvinehbaghi S, Norouzi M (2014) Electrocatalytic oxidation of formaldehydeon Ni/poly (N,N_dimethylaniline) (sodium dodecylsulfate) modified carbon paste electrode in alkaline medium. Russ J Electrochem 50:1020–1026CrossRefGoogle Scholar
  35. 35.
    Ojani R, Raoof JB, Norouzi B (2010) Carbon paste electrode modified by cobalt ions dispersed into poly (N-methylaniline) preparing in the presence of SDS: application in electrocatalytic oxidation of hydrogen peroxide. J Solid State Electrochem 14:621–631CrossRefGoogle Scholar
  36. 36.
    Ojani R, Raoof JB, Norouzi B (2011) Performance of glucose electrooxidation on Ni–Co composition dispersed on the poly(isonicotinic acid) (SDS) film. J Solid State Electrochem 15:1139–1147CrossRefGoogle Scholar
  37. 37.
    Norouzi B, Norouzi M (2012) Methanol electrooxidation on novel modified carbon paste electrodes with supported poly(isonicotinic acid) (sodium dodecyl sulfate)/Ni-Co electrocatalysts. J Solid State Electrochem 16:3003–3010CrossRefGoogle Scholar
  38. 38.
    Bard AJ, Faulkner LR (2001) Electrochemical methods: fundamentals and applications, 2nd edn. John Wiley & Sons, Inc., New YorkGoogle Scholar
  39. 39.
    Vittal R, Gomathi H, Rao GP (2001) Derivatised nickel and cobalt oxide modified electrodes: effect of surfactant. J Electroanal Chem 497:47–54CrossRefGoogle Scholar
  40. 40.
    Sun CY, Zhu YG, Zhu TJ, Xie J, Cao GS, Zhao XB (2013) Co(OH)2/graphene sheet-on-sheet hybrid as high-performance electrochemical pseudocapacitor electrodes. J Solid State Electrochem 17:1159–1165CrossRefGoogle Scholar
  41. 41.
    Liang Y, Bao S, Li H (2007) Nanocrystalline nickel cobalt hydroxides/ultrastable Y zeolite composite for electrochemical capacitors. J Solid State Electrochem 11:571–576CrossRefGoogle Scholar
  42. 42.
    Salimi A, Hallaj R, Soltanian S, Mamkhezri H (2007) Nanomolar detection of hydrogen peroxide on glassy carbon electrode modified with electrodeposited cobalt oxide nanoparticles. Anal Chim Acta 594:24–31CrossRefGoogle Scholar
  43. 43.
    Norouzi B, Parsa Z (2018) Determination of sulfite in real sample by an electrochemical sensor based on Ni/poly (4-aminobenzoic acid)/SDS/CPE. Russ J Electrochem (in press)Google Scholar
  44. 44.
    Hosseini H, Mahyari M, Bagheri A, Shaabani A (2014) A novel bioelectrochemical sensing platform based on covalently attachment of cobalt phthalocyanine to grapheme oxide. Biosens Bioelectron 52:136–142CrossRefGoogle Scholar
  45. 45.
    Abbas MN, Saeed AA, Singh B, Radowan AA, Dempsey E (2015) A cysteine sensor based on a gold nanoparticle-iron phthalocyaninemodified graphite paste electrode. Anal Methods 7:2529–2536CrossRefGoogle Scholar
  46. 46.
    Gulppi MA, Paez MA, Costamagna JA, Cardenas-Jiron G, Bedioui F, Zagal JH (2005) Inverted correlations between rate constants and redox potential of the catalyst for the electrooxidation of 2-aminoethanethiol mediated by surface confined substituted cobalt-phthalocyanines. J Electroanal Chem 580:50–56CrossRefGoogle Scholar
  47. 47.
    Griveau S, Albin V, Pauporte T, Zagal JH, Bedioui F (2002) Comparative study of electropolymerized cobalt porphyrin and phthalocyanine based films for the electrochemical activation of thiols. J Mater Chem 12:225–232CrossRefGoogle Scholar
  48. 48.
    Zagal JH, Griveau S, Francisco Silva J, Nyokong T, Bedioui F (2010) Metallophthalocyanine based molecular materials as catalysts for electrochemical reactions. Coord Chem Rev 254:2755–2791CrossRefGoogle Scholar
  49. 49.
    Hernandez-Ibanez N, Sanjuan I, Montiel MA, Foster CW, Banks CE, Iniesta J (2016) L-cysteine determination in embryo cell culture media using co (II)-phthalocyanine modified disposable screen-printed electrodes. J Electroanal Chem 780:303–310CrossRefGoogle Scholar
  50. 50.
    Teixeira MFS, Dockal ER, Cavalheiro ETG (2005) Sensor for cysteine based on oxovanadium(IV) complex of Salen modified carbon paste electrode. Sen Act B 106:619–625CrossRefGoogle Scholar
  51. 51.
    Amini MK, Khorasani JH, Khaloo SS, Tangestaninejad S (2003) Cobalt(II) salophen-modified carbon-paste electrode for potentiometric and voltammetric determination of cysteine. Anal Biochem 320:32–38CrossRefGoogle Scholar
  52. 52.
    Kohilarani K, Chen SM, Devasenathipathy R, Wang SF (2017) A simple fabrication of Co(II)-phthalocyamine modified disposable activated screen printed carbon electrode for the effective determination of L-cysteine. Int J Electrochem Sci 12:1550–1560CrossRefGoogle Scholar
  53. 53.
    Xu H, Xiao J, Liu B, Grivea S, Bedioui F (2015) Enhanced electrochemical sensing of thiols based on cobalt phthalocyanine immobilized on nitrogen-doped graphene. Biosens Bioelectron 66:38–444Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Qaemshahr BranchIslamic Azad UniversityQaemshahrIran

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