Amperometric determination of diazinon by gold nanorods/ds-DNA/graphene oxide sandwich-modified electrode
- 134 Downloads
Diazinon (DZ) is an organophosphorus pesticide which is used as an insecticide. Because of its harmful effects, finding a sensitive method to determination of DZ is important. So, in this study, a chemically modified electrode by graphene oxide (GO), functionalized double-strand DNA (ds-DNA) and gold nanorods (GNRs) was used. Interaction between oxygenated groups of GO and GNRs with amine-thiol groups of ds-DNA was used to construct a sandwich-modified electrode named GNRs/ds-DNA/GO/GCE. Characterization of synthesized GNRs and GO was done by transmission electron microscopy and scanning electron microscopy. To finding the effects of modifiers on DZ detection, cyclic voltammetry, and electrochemical impedance spectroscopy were used in each modification steps. The pH and scan rate were optimized at 6.0 and 0.1 V s−1, respectively. Dynamic range of GNRs/ds-DNA/GO/GCE in DZ determination was studied by amperometry with the concentration linear range of 1.9 × 10−6 to 5.6 × 10−5 mol L−1 and detection limit of 1.9 × 10−7 mol L−1. Finally, the application of modified electrode was evaluated on two polluted river water samples with 98.5 to 101% recovery.
KeywordsDiazinon Biosensor ds-DNA Graphene oxide Gold nanorods
We gratefully acknowledge the post-graduate office of Guilan University for supporting this work and the Samet Tak Khazar laboratory for providing the equipment of this study.
Compliance with ethical standards
All procedures performed in studies were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Davoodi D, Hassanzadeh-Khayyat M, Rezaei MA, Mohajeri SA (2014) Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples. Food Chem 158:421–428CrossRefPubMedGoogle Scholar
- 2.Akhlaghi H, Motavalizadeh Kakhky AR, Emamiyan R (2013) Determination of diazinon in fruits from northeast of Iran using the QuEChERS sample preparation method and GC/MS. Asian J Chem 25:1727–1729Google Scholar
- 4.Tadeo JL (ed) (2008) Analysis of pesticides in food and environmental samples. CRC Press, Taylor & Francis Group LLC, Boca RatonGoogle Scholar
- 28.Brodie BC (1860) On the atomic weight of graphite. Ann Chim Phys 59:466–472Google Scholar