Highly sensitive electrochemical detection of adenine on a graphene-modified carbon ionic liquid electrode
- First Online:
- 458 Downloads
A new electrochemical method for the sensitive detection of adenine was established on a chitosan (CTS)- and graphene (GR)-modified carbon ionic liquid electrode (CILE). CILE was prepared by mixing 1-butylpyridinium hexafluorophosphate (BPPF6) and paraffin with graphite powder. Due to the synergistic effects of GR, CILE, and the interaction of GR with IL on the electrode surface, the electrochemical performance of CTS/GR/CILE were greatly enhanced. Electrochemical behaviors of adenine on the modified electrode was investigated with a single well-defined oxidation peak appeared. The electrochemical reaction of adenine was an adsorption-controlled irreversible process, and the electrochemical parameters were further calculated. Under the optimal conditions, the oxidation peak current was proportional to adenine concentration in the range from 1.0 nmol L−1 to 70.0 μmol L−1 with a detection limit of 0.286 nmol L−1 (3σ) by differential pulse voltammetry. The established method showed the advantages such as good selectivity, stability, and repeatability.
KeywordsAdenine Graphene 1-Butylpyridinium hexafluorophosphate Cyclic voltammetry
- 3.Dryhurst G (1977) Electrochemistry of biological molecules. Academic, New YorkGoogle Scholar
- 6.Wu K, Fei J, Bai W, Hu S (2003) Direct electrochemistry of DNA, guanine and adenine at a nanostructured film-modified electrode. Anal Bioanal Chem 376:205–209Google Scholar
- 7.Rajendra NG, Sunita B, Rakesh KS (2011) Electrochemicmal sensor for the simultaneous voltammetric determination of adenosine and adenine. Indian J Chem 50A:1026–1034Google Scholar