There are five described Rhynchosporium species, Rhynchosporium commune, R. secalis, R. agropyri, R. orthosporum and R. lolii, that cause scald diseases on Poaceae. This study used morphological (conidial shape and size) and phylogenetic analyses of two loci (the internal transcribed spacer region (ITS) and β-tubulin (TUBB)) to identify Rhynchosporium species and their host ranges in Iran. Despite the large variation observed for Rhynchosporium conidial dimensions, the phylogenetic analyses of the ITS region and concatenated ITS and TUBB loci revealed that all isolates from wild grasses in Iran belong to R. commune. R. commune was isolated from Hordeum murinum ssp. glaucum, Hordeum vulgare ssp. spontaneum, Lolium multiflorum and Avena sativa in Iran. A. sativa has only been reported from Iran as a host for R. commune. After cross inoculation, A. sativa was considered as the most resistant host showing the lowest susceptibility to R. commune isolates. Of the grass hosts tested, H. vulgare ssp. spontaneum was the most susceptible. The most aggressive isolate across all tested hosts was isolated from Hordeum murinum ssp. glaucum. Cross-infection of the R. commune isolates from all hosts onto uncultivated grasses and cultivated barley suggests the potential of the uncultivated grasses as inoculum sources for cultivated barley epidemics and pathogen evolution. Thus, management of uncultivated grasses in the vicinity of barley fields should assist in managing the disease on cultivated barley.
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Ali S, Gladieux P, Rahman H et al (2014) Inferring the contribution of sexual reproduction, migration and off-season survival to the temporal maintenance of microbial populations: a case study on the wheat fungal pathogen Puccinia striiformis f. sp. tritici. Mol Ecol 23:603–617
Ali SM (1981) Barley grass as a source of pathogenic variation in Rhynchosporium secalis. Aust J Agric Res 32:21–25
Avrova A, Knogge W (2012) Rhynchosporium commune: a persistent threat to barley cultivation. Mol Plant Pathol 13:986–997
Beigi S, Zamanizadeh H, Razavi M, Zare R (2013) Genetic diversity of Iranian isolates of barley scald pathogen (Rhynchosporium secalis) making use of molecular markers. J Agric Sci Technol 15:843–854
Black BD, Padgett GB, Russin JS et al (1996) Potential weed hosts for Diaporthe phaseolorum var. caulivora, causal agent for soybean stem canker. Plant Dis 80:763–765
Brown JS (1985) Pathogenic variation among isolates of Rhynchosporium secalis from cultivated barley growing in Victoria, Australia. Euphytica 34:129–133
Burdon JJ, Roelfs AP (1985) The effect of sexual and asexual reproduction on the isozyme structure of populations of Puccinia graminis. Phytopathology 75:1068–1073
Burdon JJ, Thrall PH (2008) Pathogen evolution across the agro-ecological interface: implications for disease management. Evol Appl 1:57–65
Caldwell RM (1937) Rhynchosporium scald of barley, rye, and other grasses. J Agric Res 55:175–198
Charrad M, Ghazzali N, Boiteau V, et al (2014) Package “NbClust.” J Stat Softw 61:1–36
Cuénoud P, Savolainen V, Chatrou LW et al (2002) Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbcL, atpB, and matK DNA sequences. Am J Bot 89:132–144
Davies DL, Bouldin DW (1979) A cluster separation measure. IEEE Trans Pattern Anal Mach Intell 1:224–227
Drumwright AM, Allen BW, Huff KA et al (2011) Survey and DNA barcoding of Poaceae in flat rock cedar glades and barrens state natural area, Murfreesboro, Tennessee. Castanea 76:300–310
Ershad J (2009) Fungi of Iran, 3rd edn. Agricultural Research, Education and Extention Organization, Tehran
Forgan AH, Knogge W, Anderson PA (2007) Asexual genetic exchange in the barley pathogen Rhynchosporium secalis. Phytopathology 97:650–654
Foster SJ, Fitt BDL (2003) Isolation and characterization of the mating-type (MAT) locus from Rhynchosporium secalis. Curr Genet 44:277–286
Frank AB (1897) Über die Zerstörung der Gerste durch einen neuen Getreidepilz. Wochenschr Brau 42:518–520
Genger RK, Nesbitt K, Brown DA et al (2005) A novel barley scald resistance gene: genetic mapping of the Rrs15 scald resistance gene derived from wild barley, Hordeum vulgare ssp. spontaneum. Plant Breed 124:137–141
Goodwin SB (2002) The barley scald pathogen Rhynchosporium secalis is closely related to the discomycetes Tapesia and Pyrenopeziza. Mycol Res 106:645–654
Heinsen E (1901) Beobachtungen uiber den neuen Getreidepilz Rhynchosporium graminicola. Jahrb Hamburg Wiss Anstalt 18:43–55
Jarosz AM, Burdon JJ (1996) Resistance to barley scald (Rhynchosporium secalis) in wild barley grass (Hordeum glaucum and Hordeum leporinum) populations in South-Eastern Australia. Aust J Agric Res 47:413–425
King KM, West JS, Brunner PC et al (2013) Evolutionary relationships between Rhynchosporium lolii sp. nov. and other Rhynchosporium species on grasses. PLoS One 8:e72536. https://doi.org/10.1371/journal.pone.0072536
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Linde CC, Smith LM, Peakall R (2016) Weeds, as ancillary hosts, pose disproportionate risk for virulent pathogen transfer to crops. BMC Evol Biol 16:101. https://doi.org/10.1186/s12862-016-0680-6
Linde CC, Smith LM (2019) Host specialisation and disparate evolution of Pyrenophora teres f. teres on barley and barley grass. BMC Evol Biol 19:139. https://doi.org/10.1186/s12862-019-1446-8
Matlab R (2013) Version 8.1. 0.604 (R2013a). Natrick, Massachusetts MathWorks Inc.
Mourelos CA, Malbrán I, Balatti PA et al (2014) Gramineous and non-gramineous weed species as alternative hosts of Fusarium graminearum, causal agent of Fusarium head blight of wheat, in Argentina. Crop Prot 65:100–104
Mułenko W, Majewski T, Ruszkiewicz-Michalska M (2008) A preliminary checklist of micromycetes in Poland. Polish Academy of Sciences, Poland
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4326
Otsu N (1979) A threshold selection method from gray-level histograms. IEEE Trans Syst Man Cybern 9:62–66
Oudemans C (1897) Observations mycologiques. Konink Akad Wetensch Amsterdam 6:86–92
Seifollahi E, Sharifnabi B, Javan-Nikkhah M, Linde CC (2018) Low genetic diversity of Rhynchosporium commune in Iran, a secondary Centre of barley origin. Plant Pathol 67:1725–1734
Shipton WA, Boyd WJR, Ali SM (1974) Scald of barley. Rev Plant Pathol 53:839–861
Team RDC (2011) R development Core team: R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
Welty RE, Metzger R (1996) First report of scald of triticale caused by Rhynchosporium secalis in North America. Plant Dis 80:1220–1223
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc 18:315–322
Wilkins P (1973) Infection of Lolium multifiorum with Rhynchosporium species. Plant Pathol 22:107–111
Zaffarano PL, McDonald BA, Linde CC (2011) Two new species of Rhynchosporium. Mycologia 103:195–202
Zaffarano PL, McDonald BA, Linde CC (2008) Rapid speciation following recent host shifts in the plant pathogenic fungus Rhynchosporium. Evolution 62:1418–1436
E.S. thanks Isfahan University of Technology for financial support. The authors thank S. Beigi, J. Shokri and A. Ahmadpour who contribute during sampling. Also, we thank J. Gholami for technical assistance, L. Smith for guidance in pathogenicity test, S. Taghadomi-Saberi for image processing, M.R. Sabzalian and M. Isapareh for guidance with statistical analysis.
This study was funded by Isfahan University of Technology (grant number NA).
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Seifollahi, E., Sharifnabi, B., Javan-Nikkhah, M. et al. Scald on gramineous hosts in Iran and their potential threat to cultivated barley. Mycol Progress 19, 223–233 (2020). https://doi.org/10.1007/s11557-019-01553-8
- Host specificity