Milesina thailandica, a second rust fungus on an early diverged leptosporangiate fern genus, Lygodium, found in Thailand

Mycological Progress volume 19, pages147–154(2020)Cite this article

Abstract

A new rust fungus was found on Lygodium flexuosum at Doi Suthep-Pui National Park in Chiang Mai Province, Thailand. Puccinia lygodii was the only rust fungus currently known on Lygodium spp. (Lygodioideae, Schizaeaceae, Schizaeales) from southern North America through northern South America. The new fungus produced uredinia linearly between veins, causing yellowish streaks, on the abaxial frond surface. The sori were covered with a layer of thin-walled fungal cells beneath the host epidermis and surrounded by thin-walled paraphyses. The sori did not have a distinct central aperture with ostiolar cells but ruptured irregularly to release urediniospores. Urediniospores were obovoid, obovoid-ellipsoid, or pyriform. The wall was thin, colorless, and completely echinulate. Neither amphispores nor teliospores were observed. The sorus and spore morphology indicated the taxonomic affinity of the fungus to the fern rust genera Hyalopsora and Milesina. A molecular phylogenetic analysis using nuclear large subunit ribosomal DNA sequence revealed that the Lygodium rust fungus was grouped in a paraphyletic Milesina clade. From the putative host preference, morphology, and estimated phylogenetic relationships, it was concluded that the fungus was a new species of Milesina.

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Acknowledgments

This study was carried out under the permission from the National Research Council of Thailand for the international project entitled “Taxonomy and life cycle of rust fungi with special reference to grapevine and fern leaf rusts” (Project ID: 2018/043). We thank the Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, Thailand, for permitting to survey plant rusts and to sample rusted plant leaves at Doi Suthep-Pui National Park.

The Forest Herbarium (the Department of National Park, Wildlife and Plant Conservation) provided us with the opportunity to examine Lygodium specimens for search of possible rust infection.

Funding

This study was supported, in part, by a Grant-in-Aid (G-2018-1-019) from the Institute of Fermentation, Osaka (IFO) (YO), and a Grant-in-Aid for Scientific Research (A) No. 14255004 from the Ministry of Education, Science and Culture, Japan (YO).

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Affiliations

  1. Faculty of Education, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki, 310-8512, Japan

    Yoshitaka Ono

  2. Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom, 73140, Thailand

    Yoshitaka Ono, Jintana Unartngam & Pattama Janruang

  3. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Katsura Ohmachi & Izumi Okane

  4. Department of National Park, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment, Bangkok, 110900, Thailand

    Chanjira Ayawong

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  1. Yoshitaka Ono
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  2. Katsura Ohmachi
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Correspondence to Yoshitaka Ono.

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Ono, Y., Ohmachi, K., Unartngam, J. et al. Milesina thailandica, a second rust fungus on an early diverged leptosporangiate fern genus, Lygodium, found in Thailand. Mycol Progress 19, 147–154 (2020). https://doi.org/10.1007/s11557-019-01549-4

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Keywords

  • Geographic distribution
  • Host specificity
  • Lygodium flexuosum
  • Phylogeny
  • Pucciniales
  • Schizaeaceae