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Mycological Progress

, Volume 18, Issue 11, pp 1329–1339 | Cite as

Pseudoplagiostoma myracrodruonis (Pseudoplagiostomataceae, Diaporthales): a new endophytic species from Brazil

  • Jadson D. P. BezerraEmail author
  • Ana P. S. L. Pádua
  • Thays G. L. Oliveira
  • Laura M. Paiva
  • Vladimiro Guarnaccia
  • Xinlei Fan
  • Cristina M. Souza-MottaEmail author
Original Article

Abstract

Two initially unidentifiable isolates were found when endophytic fungi from healthy leaves of Myracrodruon urundeuva (Anacardiaceae) were being investigated in Brazil. Based on phylogenetic analyses using internal transcribed spacer (ITS) and partial large subunit (LSU) of ribosomal DNA, RNA polymerase II (RPB2), translation elongation factor EF-1 alpha (TEF1), and tubulin (TUB2), the isolates were identified as members of the family Pseudoplagiostomataceae (Diaporthales). In this paper, we introduce a new species, Pseudoplagiostoma myracrodruonis, thus accepting eight species in Pseudoplagiostoma (Pseudoplagiostomataceae). The new species is proposed to accommodate endophytes characterized by a coelomycetous asexual morph, pycnidial conidiomata with lageniform to ampulliform and hyaline to pale brown phialidic conidiogenous cells that produce unicellular, ellipsoid to oblong-cylindrical, hyaline, and guttulate conidia. In the current phylogenetic analysis, P. myracrodruonis is placed in a well-supported clade in Pseudoplagiostoma (Pseudoplagiostomataceae). A discussion about the relationship of Pseudoplagiostoma species is included in the paper.

Keywords

Ascomycota Diaporthalean fungi Endophytes Fungal taxonomy Phylogeny Sordariomycetes 

Notes

Acknowledgements

We value all the observations made by the two anonymous reviewers for this paper.

Funding information

The authors and this research received fellowships and financial support, respectively, from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Finance Code 001), the Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE), and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

References

  1. Alvarez LV, Groenewald JZ, Crous PW (2016) Revising the Schizoparmaceae: Coniella and its synonyms Pilidiella and Schizoparme. Stud Mycol 85:1–34.  https://doi.org/10.1016/j.simyco.2016.09.001 CrossRefPubMedPubMedCentralGoogle Scholar
  2. Bezerra JDP, Santos MGS, Barbosa RN, Svedese VM, Lima DMM, Fernandes MJS, Gomes BS, Paiva LM, Almeida-Cortez JS, Souza-Motta CM (2013) Fungal endophytes from cactus Cereus jamacaru in Brazilian tropical dry forest: a first study. Symbiosis 60:53–63.  https://doi.org/10.1007/s13199-013-0243-1 CrossRefGoogle Scholar
  3. Bezerra JDP, Oliveira RJV, Paiva LM, Silva GA, Groenewald JZ, Crous PW, Souza-Motta CM (2017a) Bezerromycetales and Wiesneriomycetales ord. nov. (class Dothideomycetes), with two novel genera to accommodate endophytic fungi from Brazilian cactus. Mycol Prog 16:297–309.  https://doi.org/10.1007/s11557-016-1254-0 CrossRefGoogle Scholar
  4. Bezerra JDP, Sandoval-Denis M, Paiva LM, Silva GA, Groenewald JZ, Souza-Motta CM, Crous PW (2017b) New endophytic Toxicocladosporium species from cacti in Brazil, and description of Neocladosporium gen. nov. IMA Fungus 8:77–97.  https://doi.org/10.5598/imafungus.2017.08.01.06 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Braun U, Nakashima C, Crous PW, Groenewald JZ, Moreno-Rico O, Rooney-Latham S, Blomquist CL, Haas J, Marmolejo J (2018) Phylogeny and taxonomy of the genus Tubakia s. lat. Fungal Syst Evol 1:41–99.  https://doi.org/10.3114/fuse.2018.01.04 CrossRefGoogle Scholar
  6. Brum MCP, Araújo WL, Maki CS, Azevedo JL (2012) Endophytic fungi from Vitis labrusca L. (‘Niagara Rosada’) and its potential for the biological control of Fusarium oxysporum. Genet Mol Res 11:4187–4197.  https://doi.org/10.4238/2012.December.6.2 CrossRefPubMedGoogle Scholar
  7. Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556.  https://doi.org/10.2307/3761358 CrossRefGoogle Scholar
  8. Carvalho CR, Ferreira-D’Silva A, Wedge DE, Cantrell CL, Rosa LH (2018) Antifungal activities of cytochalasins produced by Diaporthe miriciae, an endophytic fungus associated with tropical medicinal plants. Can J Microbiol 64:835–843.  https://doi.org/10.1139/cjm-2018-0131 CrossRefPubMedGoogle Scholar
  9. Cheewangkoon R, Groenewald JZ, Verkley GJM, Hyde KD, Wingfield MJ, Gryzenhout M, Summerell BA, Denman S, Toanun C, Crous PW (2010) Re-evaluation of Cryptosporiopsis eucalypti and cryptosporiopsis-like species occurring on Eucalyptus leaves. Fungal Divers 44:89–105.  https://doi.org/10.1007/s13225-010-0041-5 CrossRefGoogle Scholar
  10. Costa IPW, Maia LC, Cavalcanti MA (2012) Diversity of leaf endophytic fungi in mangrove plants of Northeast Brazil. Braz J Microbiol 43:1165–1173.  https://doi.org/10.1590/S1517-838220120003000044 CrossRefGoogle Scholar
  11. Crous PW et al (2012) A re-appraisal of Harknessia (Diaporthales), and the introduction of Harknessiaceae fam. nov. Persoonia 28:49–65.  https://doi.org/10.3767/003158512X639791 CrossRefPubMedPubMedCentralGoogle Scholar
  12. Crous PW et al (2016a) Fungal Planet description sheets: 469–557. Persoonia 37:218–403.  https://doi.org/10.3767/003158516X694499 CrossRefPubMedPubMedCentralGoogle Scholar
  13. Crous PW, Groenewald JZ, Slippers B, Wingfield MJ (2016b) Global food and fibre security threatened by current inefficiencies in fungal identification. Philos Trans R Soc B 371:20160024.  https://doi.org/10.1098/rstb.2016.0024 CrossRefGoogle Scholar
  14. Crous PW et al (2018) Fungal Planet description sheets: 716–784. Persoonia 41:238–417.  https://doi.org/10.3767/persoonia.2018.41.12 CrossRefPubMedPubMedCentralGoogle Scholar
  15. Crous PW, Schumacher RK, Akulov A, Thangavel R, Hernández-Restrepo M, Carnegie AJ, Cheewangkoon R, Wingfield MJ, Summerell BA, Quaedvlieg W, Coutinho TA, Roux J, Wood AR, Giraldo A et al (2019) New and interesting fungi. 2. Fungal Syst Evol 3:57–134.  https://doi.org/10.3114/fuse.2019.03.06 CrossRefGoogle Scholar
  16. Du Z, Hyde KD, Yang Q, Liang YM, Tian CM (2017) Melansporellaceae: a novel family of Diaporthales (Ascomycota). Phytotaxa 305:191–200.  https://doi.org/10.11646/phytotaxa.305.3.6 CrossRefGoogle Scholar
  17. Fan XL, Bezerra JDP, Tian CM, Crous PW (2018a) Families and genera of diaporthalean fungi associated with canker and dieback of tree hosts. Persoonia 40:119–134.  https://doi.org/10.3767/persoonia.2018.40.05 CrossRefGoogle Scholar
  18. Fan XL, Du Z, Bezerra JDP, Tian CM (2018b) Taxonomic circumscription of Melanconis-like fungi causing canker disease in China. MycoKeys 42:89–124.  https://doi.org/10.3897/mycokeys.42.29634 CrossRefGoogle Scholar
  19. Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330PubMedPubMedCentralGoogle Scholar
  20. Gomes RR, Glienke C, Videira SIR, Lombard L, Groenewald JZ, Crous PW (2013) Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 31:1–41.  https://doi.org/10.3767/003158513X666844 CrossRefPubMedPubMedCentralGoogle Scholar
  21. Guarnaccia V, Vitale A, Cirvilleri G, Aiello D, Susca A, Epifani F, Polizzi G (2016) Characterisation and pathogenicity of fungal species associated with branch cankers and stem-end rot of avocado in Italy. Eur J Plant Pathol 146:963–976.  https://doi.org/10.1007/s10658-016-0973-z CrossRefGoogle Scholar
  22. Guarnaccia V, Groenewald JZ, Woodhall J, Armengol J, Cinelli T, Eichmeier A, Ezra D, Fontaine F, Gramaje D, Gutierrez-Aguirregabiria A, Kaliterna J, Kiss L, Larignon P, Luque J et al (2018) Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe. Persoonia 40:135–153.  https://doi.org/10.3767/persoonia.2018.40.06 CrossRefPubMedPubMedCentralGoogle Scholar
  23. Guterres DC, Galvão-Elias S, Santos MDM, Souza BCP, Almeida CP, Pinho DB, Miller RNG, Dianese JC (2019) Phylogenetic relationships of Phaeochorella parinarii and recognition of a new family, Phaeochorellaceae (Diaporthales). Mycologia 111:660–675.  https://doi.org/10.1080/00275514.2019.1603025 CrossRefPubMedGoogle Scholar
  24. Hyde KD, Soytong K (2008) The fungal endophyte dilemma. Fungal Divers 33:163–173Google Scholar
  25. Jeewon R, Hyde KD (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7:1669–1677.  https://doi.org/10.5943/mycosphere/7/11/4 CrossRefGoogle Scholar
  26. Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780.  https://doi.org/10.1093/molbev/mst010 CrossRefPubMedPubMedCentralGoogle Scholar
  27. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874.  https://doi.org/10.1093/molbev/msw054 CrossRefPubMedPubMedCentralGoogle Scholar
  28. Murali TS, Suryanarayanan TS, Venkatesan G (2007) Fungal endophytes communities in two tropical forest of southern India: diversity and host affiliation. Mycol Prog 6:191–199.  https://doi.org/10.1007/s11557-007-0540-2 CrossRefGoogle Scholar
  29. Nylander JAA (2004) MrModeltest 2.2. Computer program and documentation distributed by the author. Evolutionary Biology Centre, Uppsala University, UppsalaGoogle Scholar
  30. O’Donnell K, Sutton DA, Rinaldi MG, Sarver BA, Balajee SA, Schroers HJ, Summerbell RC, Robert VA, Crous PW, Zhang N, Aoki T, Jung K, Park J, Lee YH, Kang S, Park B, Geiser DM (2010) Internet-accessible DNA sequence database for identifying fusaria from human and animal infections. J Clin Microbiol 48:3708–3718.  https://doi.org/10.1128/JCM.00989-10 CrossRefPubMedPubMedCentralGoogle Scholar
  31. Old KM, Wingfield MJ, Yuan ZQ (2003) Cryptosporiopsis leaf blight: a manual of diseases of Eucalyptus in South-East Asia. CIFOR and ACIAR, Bogor, pp 10–13Google Scholar
  32. Oliveira RJV, Bezerra JL, Lima TEF, da Silva GA, Cavalcanti MAQ (2016) Phaeosphaeria nodulispora, a new endophytic coelomycete isolated from tropical palm (Cocos nucifera) in Brazil. Nova Hedwigia 103:185–192.  https://doi.org/10.1127/nova_hedwigia/2016/0343 CrossRefGoogle Scholar
  33. Pádua APSL, Freire KTLS, Oliveira TGL, Silva LF, Araújo-Magalhães GR, Agamez-Montalvo GS, Silva IR, Bezerra JDP, Souza-Motta CM (2019) Fungal endophyte diversity in the leaves of the medicinal plant Myracrodruon urundeuva in a Brazilian dry tropical forest and their capacity to produce L-asparaginase. Acta Bot Bras 33:39–49.  https://doi.org/10.1590/0102-33062018abb0108 CrossRefGoogle Scholar
  34. Phookamsak R, Hyde KD, Jeewon R, Bhat DJ, Jones EBG, Maharachchikumbura S, Raspé O, Karunarathna SC, Wanasinghe DN, Hongsanan S et al (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Divers 95:1–273.  https://doi.org/10.1007/s13225-019-00421-w CrossRefGoogle Scholar
  35. Rayner RW (1970) A mycological colour chart. CMI and British Mycological Society, KewGoogle Scholar
  36. Rossman AY, Farr DF, Castlebury LA (2007) A review of the phylogeny and biology of the Diaporthales. Mycoscience 48:135–144.  https://doi.org/10.1007/s10267-007-0347-7 CrossRefGoogle Scholar
  37. Santos TT, Leite TS, Queiroz CB, Araújo EF, Pereira OL, Queiroz MV (2016) High genetic variability in endophytic fungi from the genus Diaporthe isolated from common bean (Phaseolus vulgaris L.) in Brazil. J Appl Microbiol 120:388–401.  https://doi.org/10.1111/jam.12985 CrossRefPubMedGoogle Scholar
  38. Sebastianes FLS, Romão-Dumaresq AS, Lacava PT, Harakava R, Azevedo JL, de Melo IS, Pizzirani-Kleiner AA (2013) Species diversity of culturable endophytic fungi from Brazilian mangrove forests. Curr Genet 59:153–166.  https://doi.org/10.1007/s00294-013-0396-8 CrossRefGoogle Scholar
  39. Senanayake IC, Crous PW, Groenewald JZ, Maharachchikumbura SSN, Jeewon R, Phillips AJL, Bhat JD, Perera RH, Li QR, Li WJ, Tangthirasunun N, Norphanphoun C, Karunarathna SC, Camporesi E, Manawasighe IS, Al-Sadi AM, Hyde KD (2017) Families of Diaporthales based on morphological and phylogenetic evidence. Stud Mycol 86:217–296.  https://doi.org/10.1016/j.simyco.2017.07.003 CrossRefPubMedPubMedCentralGoogle Scholar
  40. Senanayake IC, Jeewon R, Chomnunti P, Wanasinghe DN, Norphanphoun C, Karunarathna A, Pem D, Perera RH, Camporesi E, McKenzie EHC, Hyde KD, Karunarathna SC (2018) Taxonomic circumscription of Diaporthales based on multigene phylogeny and morphology. Fungal Divers 93:241–443.  https://doi.org/10.1007/s13225-018-0410-z CrossRefGoogle Scholar
  41. Silva RMF, Oliveira RJV, Bezerra JDP, Bezerra JL, Souza-Motta CM, Silva GA (2019) Bifusisporella sorghi gen. et sp. nov. (Magnaporthaceae) to accommodate an endophytic fungus from Brazil. Mycol Prog 18:847–854.  https://doi.org/10.1007/s11557-019-01494-2 CrossRefGoogle Scholar
  42. Siqueira VM, Braun U, Souza-Motta CM (2008) Corynespora subcylindrica sp. nov., a new hyphomycete species from Brazil and a discussion on the taxonomy of corynespora-like genera. Sydowia 60:113–122Google Scholar
  43. Stamatakis A, Hoover P, Rougemont J (2008) A rapid bootstrap algorithm for the RAxML web servers. Syst Biol 57:758–771.  https://doi.org/10.1080/10635150802429642 CrossRefPubMedGoogle Scholar
  44. Suwannarach N, Kumla J, Lumyong S (2016) Pseudoplagiostoma dipterocarpi sp. nov., a new endophytic fungus from Thailand. Mycoscience 57:118–122.  https://doi.org/10.1016/j.myc.2015.12.002 CrossRefGoogle Scholar
  45. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246.  https://doi.org/10.1128/jb.172.8.4238-4246.1990 CrossRefPubMedPubMedCentralGoogle Scholar
  46. White TJ, Bruns T, Lee S, Taylor L (1990) Amplification and direct sequencing of fungal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols. A guide to methods and applications. Academic Press, Cambridge, pp 315–322Google Scholar
  47. Xavier KV, KC AN, Crous PW, Groenewald JZ, Vallad GE (2019) Dwiroopa punicae sp. nov. (Dwiroopaceae fam. nov., Diaporthales), associated with leaf spot and fruit rot of pomegranate (Punica granatum). Fungal Syst Evol 4:33–41.  https://doi.org/10.3114/fuse.2019.04.04 CrossRefGoogle Scholar
  48. Yang Y, Guo YX, Zhang YK, Wu HY, Zhang M (2016) Diaporthe henanensis sp. nov., an endophytic fungus in Ziziphus jujuba from China. Mycotaxon 131:645–652.  https://doi.org/10.5248/131.645

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Micologia Prof. Chaves BatistaUniversidade Federal de PernambucoRecifeBrazil
  2. 2.Setor de Micologia, Departamento de Biociências e Tecnologia, Instituto de Patologia Tropical e Saúde PúblicaUniversidade Federal de GoiásGoiâniaBrazil
  3. 3.Department of Agricultural, Forest and Food Sciences (DISAFA)University of TorinoGrugliasco (TO)Italy
  4. 4.Centre for Innovation in the Agro-Environmental Sector, AGROINNOVAUniversity of TorinoGrugliasco (TO)Italy
  5. 5.The Key Laboratory for Silviculture and Conservation of the Ministry of EducationBeijing Forestry UniversityBeijingChina

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