Mycological Progress

, Volume 18, Issue 12, pp 1411–1421 | Cite as

Two new species of Halophytophthora from Brazil

  • Ana L. JesusEmail author
  • Agostina V. Marano
  • Danilo R. Gonçalves
  • Gustavo H. Jerônimo
  • Carmen L. A. Pires-Zottarelli
Original Article


During a survey of oomycetes in a mangrove area of São Paulo state, Brazil, a high number of isolates of Halophytophthora and related genera were recovered from leaves and water samples. In this study, we analysed the morphology and phylogenetic relationships of the isolates of Halophytophthora along with four ex-types provided by CBS-KNAW Culture Collection. Maximum likelihood and Bayesian analyses of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA regions confirmed previous evidence of the polyphyly of the genus and revealed the presence of two yet undescribed species. The two new species, Halophytophthora souzae and H. insularis, belong to the Halophytophthora sensu stricto clade and are described herein based on their morphology and/or phylogenetic position.


ITS LSU Mangroves Oomycota Peronosporales 



We are indebted to “Instituto Florestal” for the permission given to collect samples at “Ilha do Cardoso”, Cananéia, São Paulo, SP; to Manoel Osorio Neves Junior for his valuable help during sampling and to Marcela Castilho Boro for preparing dried cultures and depositing the isolates in the CCIBt culture collection.

Funding information

We also wish to thank São Paulo Research Foundation – FAPESP for the fellowships given to A.L. Jesus (Process No. 2013/01409-0) and for the financial support given to C.L.A. Pires-Zottarelli (Process No. 2012/50222-7), and CAPES (“Coordenação de Aperfeiçoamento de Pessoal de Nível Superior”) for the fellowship and support given to A.V. Marano (“Ciência Sem Fronteiras” Program, “Atração de Jovens Talentos” DRI-CAPES Process No. 006/2012). CNPq (“Conselho Nacional de Desenvolvimento Científico e Tecnológico”) is also acknowledged for the grant given to C.L.A. Pires-Zottarelli (Process No. 304411/2012-4).


  1. Anastasiou CJ, Churchland LM (1969) Fungi on decaying leaves in marine habitats. Can J Botany 47:251–257CrossRefGoogle Scholar
  2. Bazinet AL, Cummings MP (2008) The lattice project: a grid research and production environment combining multiple grid computing models. In: Weber MHW (ed) Distributed and grid computing – science made transparent for everyone. Principles, applications and supporting communities, Marburg, Germany, pp 2–13Google Scholar
  3. Bennett RM, de Cock AWAM, Lévesque CA, Thines M (2017) Calycofera gen. nov., an estuarine sister taxon to Phytopythium, Peronosporaceae. Mycol Prog 16:947–954CrossRefGoogle Scholar
  4. de Cock AWAM, Lodhi AM, Rintoul L, Bala K, Robideau GP, Abad ZG, Coffey MD, Shahzad S, Lévesque CA (2015) Phytopythium: molecular phylogeny and systematics. Persoonia 34:25–39CrossRefGoogle Scholar
  5. Fell JW, Master IM (1975) Phycomycetes (Phytophthora spp. nov and Pythium sp. nov.) associated with decaying mangrove (Rhizophora mangle) leaves. Can J Botany 53:2908–2922CrossRefGoogle Scholar
  6. Gerrettson-Cornell L, Simpsom J (1984) Three new marine Phytophthora species from New South Wales. Mycotaxon 19:453–470Google Scholar
  7. Ho HH, Jong SC (1990) Halophytophthora, gen. nov., a new member of the family Pythiaceae. Mycotaxon 36:377–382Google Scholar
  8. Ho HH, Nakagiri A, Newell SY (1992) A new species of Halophytophthora from Atlantic and Pacific subtropical islands. Mycologia 84:548–554CrossRefGoogle Scholar
  9. Ho HH, Chang HS, Huang SH (2003) Halophytophthora elongata, a new marine species from Taiwan. Mycotaxon 85:417–422Google Scholar
  10. Hulvey J, Telle S, Nigrelli L, Lamour K, Thines M (2010) Salisapiliaceae – a new family of oomycetes from marsh grass litter of southeastern North America. Persoonia 25:109–116CrossRefGoogle Scholar
  11. Jesus AL, Marano AV, Jêronimo GH, de Souza JI, Leaño EM, Pires-Zottarelli CLA (2016) The genus Halophytophthora (Peronosporales, Straminipila) in Brazil: first descriptions of species. Braz J Bot 39:729–739CrossRefGoogle Scholar
  12. Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772–780CrossRefGoogle Scholar
  13. Lara E, Belbahri L (2011) SSU rRNA reveals major trends in oomycete evolution. Fungal Divers 49:93–100CrossRefGoogle Scholar
  14. Leaño EM, Vrijmoed LLP, Jones ERG (1998) Physiological studies on Halophytophthora vesicula (straminipilous fungi) isolated from fallen mangrove leaves from Mai Po, Hong Kong. Bot Mar 41:411–419CrossRefGoogle Scholar
  15. Li GJ, Hyde KD, Zhao RL, Hongsanan S, Abdel-Aziz FA, Abdel-Wahab MA, Alvarado P et al (2016) Fungal diversity notes 253–366: taxonomic and phylogenetic contributions to fungal taxa. Fungal Divers 78:1–237CrossRefGoogle Scholar
  16. Marano AV, Jesus AL, de Souza JI, Leãno EM, James TY, Jerônimo GH, de Cock AWAW, Pires-Zottarelli CLA (2014a) A new combination in Phytopythium: P. kandeliae (Oomycetes, Straminipila). Mycosphere 5:510–522CrossRefGoogle Scholar
  17. Marano AV, Jesus AL, de Souza JI, Jerônimo GH, Pires-Zottarelli CLA (2014b) On the status of P. kandeliae (Oomycetes, Straminipila). Mycosphere 5(6):768–769CrossRefGoogle Scholar
  18. Marano AV, Jesus AL, de Souza JI, Jerônimo GH, Gonçalves DR, Boro MC, Rocha SCO, Pires-Zottarelli CLA (2015) Ecological roles of saprotrophic Peronosporales (Oomycetes, Straminipila) in natural environments. Fungal Ecol 19:77–88CrossRefGoogle Scholar
  19. Nakagiri A (2000) Ecology and diversity of Halophytophthora species. Fungal Divers 5:153–164Google Scholar
  20. Nakagiri A, Tokumasu S, Araki H, Koreeda S, Tubaki K (1989) Succession of fungi in decomposing mangrove leaves in Japan. In: Hattori T, Ishida Y, Maruyama Y, Morita R, Uchida A (eds) Recent advances in microbial ecology. Japan Science Society Press, Japan, pp 297–301Google Scholar
  21. Nakagiri A, Ito T, Monoch L, Tanticharoen M (2001) A new Halophytophthora species, H. porrigovesica, from subtropical and tropical mangrove. Mycoscience 42:33–41CrossRefGoogle Scholar
  22. Newell SY, Fell JW (1992) Distribution and experimental responses to substrate of marine oomycetes (Halophytophthora spp) in mangrove ecosystem. Mycol Res 96:851–856CrossRefGoogle Scholar
  23. Newell SY, Miller JD, Fell JW (1987) Rapid and pervasive occupation of fallen mangrove leaves by a marine zoosporic fungus. Appl Environ Microb 53:2464–2469Google Scholar
  24. Nigrelli L, Thines M (2013) Tropical oomycetes in the German Bight – climate warming or overlooked diversity? Fungal Ecol 6:152–160CrossRefGoogle Scholar
  25. Pelage L, Domalain G, Lira AS, Travasssos P, Frédou T (2019) Coastal land use in Northeast Brazil: mangrove coverage evolution over three decades. Trop Conserv Sci 12:1–15Google Scholar
  26. Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256CrossRefGoogle Scholar
  27. Riethmüller A, Voglmayr H, Göker M, Weiß M, Oberwinkler F (2002) Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. Mycologia 94:834–849CrossRefGoogle Scholar
  28. Robideau GP, de Cock AWAM, Coffey MD, Voglmayr H, Brouwer H, Bala K, Chitty DW, Désaulniers N, Eggertson QA, Gachon CMM, Hu CH, Küpper FC, Rintoul TL, Sarhan E, Verstappen ECP, Zhang Y, Bonants PJM, Ristaino JB, Lévesque AC (2011) DNA barcoding of oomycetes with cytochrome c oxidase subunit I and internal transcribed spacer. Mol Ecol Res 11:1002–1011CrossRefGoogle Scholar
  29. Ronquist F, Huelsenbeck JP (2003) Mrbayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574CrossRefGoogle Scholar
  30. Tan TK, Pek CL (1997) Tropical mangrove leaf litter fungi in Singapore with an emphasis on Halophytophthora. Mycol Res 101:165–168CrossRefGoogle Scholar
  31. Thines M (2014) Phylogeny and evolution of plant pathogenic oomycetes - a global overview. Eur J Plant Pathol 138:431–447CrossRefGoogle Scholar
  32. Yang X, Hong CX (2014) Halophytophthora fluviatilis sp. nov. from freshwater in Virginia. FEMS Microbiol Lett 352:230–237CrossRefGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Ana L. Jesus
    • 1
    Email author
  • Agostina V. Marano
    • 1
  • Danilo R. Gonçalves
    • 1
  • Gustavo H. Jerônimo
    • 1
  • Carmen L. A. Pires-Zottarelli
    • 1
  1. 1.Núcleo de Pesquisa em MicologiaInstituto de BotânicaSão PauloBrazil

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