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Mycological Progress

, Volume 18, Issue 9, pp 1121–1134 | Cite as

Sugarcane glycoproteins control dynamics of cytoskeleton during teliospore germination of Sporisorium scitamineum

  • Elena Sánchez-Elordi
  • František Baluška
  • Carlos Vicente
  • María Estrella LegazEmail author
Original Article
  • 92 Downloads

Abstract

Sporisorium scitamineum teliospores possess an organized cytoskeleton involved in important developmental and physiological processes. It has been described that microtubules appear to be fundamental for nucleus translocation during germination and hyphal growth, whereas actin polymerization is necessary for the formation of invaginations during teliospore displacement. Here, a global vision of the actin cytoskeleton organization throughout the life cycle of S. scitamineum cells is shown, providing evidence that a perfectly structured F-actin network is necessary to trigger smut pathogenicity. Moreover, although myosin presence in teliospores had been previously described, herein actin and myosin co-locations are demonstrated by confocal microscopy during both invaginations formation and germination. In turn, F-actin and microtubules (MTs) interact, jointly participating in the establishment of cell polarity. The resistant sugarcane cultivar Mayari 55-14 produces high molecular mass glycoproteins (HMMG) that differently affect F-actin organization at different stages of fungal development. HMMG first supported F-actin to induce the movement of teliospores towards the cytoagglutination points. At later stages of fungal development, HMMG disorganized F-actin which prevented the protrusion of germinative tube. A continuous exposure to HMMG provoked apoptosis in pathogenic, diploid cells and a delay in sporidia conjugation that could be crucial for plant resistance.

Keywords

Apoptotic rings Cytoskeleton Glycoproteins Microtubules Saccharum Smut 

Abbreviations

B

Barbados

BSA

Bovine serum albumin

DAPI

4′,6-Diamidino-2-phenylindole dihydrochloride

HMMG

High molecular mass glycoproteins

IgG

Immunoglobulin G

Jas

Jasplakinolide

Lat A

Latrunculin A

MT

Microtubules

My

Mayarí

Noc

Nocodazole

PBS

Phosphate saline buffer

PVP

Polyvinylpyrrolidone

TEM

Transmission electron microscopy

Notes

Funding information

This work has been supported by a grant from the Complutense University (Spain) UCM, GR3/14.910081.

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Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Elena Sánchez-Elordi
    • 1
  • František Baluška
    • 2
  • Carlos Vicente
    • 1
  • María Estrella Legaz
    • 1
    Email author
  1. 1.Team of Intercellular Communication in Plant Symbiosis, Faculty of BiologyComplutense UniversityMadridSpain
  2. 2.Department of Plant Cell Biology, Institute of Cellular and Molecular Botany (IZMB)University BonnBonnGermany

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