Mycological Progress

, Volume 18, Issue 8, pp 1071–1078 | Cite as

Two new species of Clavulina (Cantharellales) from southwestern China based on morphological and molecular evidence

  • Cheng-Long Wu
  • Ya He
  • Jun Yan
  • Ping ZhangEmail author
Original Article


Two new species of Clavulina (Clavulinaceae, Cantharellales, Basidiomycota) from southwestern China are substantiated based on morphological evidence and molecular phylogenetic analyses. Both are consistent with the generic properties of Clavulina that include coralloid, branched basidiomata, amphigenous hymenia, basidia with two cornuted sterigmata, and postpartal septa. The first new species, Clavulina flava, is unique for Clavulina in having bright yellow basidiomata, and the second, Clavulina purpurascens, has pale purple basidiomata. The two new species are described in detail, illustrated with line drawings and photographs, and compared with similar species. Intrageneric relationships were examined with phylogenetic analyses based on the sequences of the second largest subunit of DNA-dependent RNA polymerase II (RPB2) and the large subunit of nuclear ribosomal RNA gene (LSU). A key is provided for the known Clavulina species in China.


Coral fungi Morphological feature Phylogenetic analysis Fungal taxonomy 


Funding information

This study was financially supported by the National Natural Science Foundation of China (No. 31750001, QYZDY-SSW-SMC029; No. 31670015), Hunan Provincial Cooperative Innovation Center of Engineering and New Products for Developmental Biology, China (Grant No. 20134486), and Hunan Provincial Construct Program of the Key Discipline in Ecology, China (No. 0713).


  1. Corner EJH (1950) A monograph of Clavaria and allied genera. Oxford University press, LondonGoogle Scholar
  2. Corner EJH (1970) Supplement to “A monograph of Clavaria and allied genera”. Beihefte zur Nova Hedwigia 33:1–299Google Scholar
  3. Douanla-Meli CM (2007) Fungi of Cameroon. Bibl Mycol 202:1–410Google Scholar
  4. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  5. Duhem B, Buyck B (2007) Edible mushrooms from Madagascar (2).Clavulina albiramea comb. nov. (Cantharellales), an edible clavarioid fungus shared between African miombo and Malagasy Uapaca woodland. Nova Hedwigia 85:317–330. CrossRefGoogle Scholar
  6. Edgar RC (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinf 5:113. CrossRefGoogle Scholar
  7. Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 2:113–118. CrossRefGoogle Scholar
  8. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  9. He G, Chen SL, Yan SZ (2016) Morphological and molecular evidence for a new species in Clavulina from southwestern China. Mycoscience 57:255–263. CrossRefGoogle Scholar
  10. Henkel TW, Aime MC, Uehling JK, Smith ME (2011) New species and distribution records of Clavulina (Cantharellales, Basidiomycota) from the Guiana Shield. Mycologia 103:883–894. CrossRefPubMedGoogle Scholar
  11. Henkel TW, Meszaros R, Aime MC, Kennedy A (2005) New Clavulina species from the Pakaraima Mountains of guyana. Mycol Prog 4:343–350. CrossRefGoogle Scholar
  12. Kornerup A, Wanscher JH (1978) Methuen handbook of colour, 3rd edn. Methuen, LondonGoogle Scholar
  13. Liu LN, Wu L, Chen ZH, Bau T, Zhang P (2017) The species of Lentaria (Gomphales, Basidiomycota) from China based on morphological and molecular evidence. Mycol Prog 16:605–612. CrossRefGoogle Scholar
  14. Moncalvo JM, Nilsson RH, Koster B, Dunham SM, Bernauer T, Matheny PB, McLenon T, Margaritescu S, Weiß M, Garnica S, Danell E, Langer G, Langer E, Larsson E, Larsson KH, Vilgalys R (2006) The cantharelloid clade: dealing with incongruent gene trees and phylogenetic reconstruction methods. Mycologia 98:937–948. CrossRefPubMedGoogle Scholar
  15. Moyersoen B (2006) Pakaraimaea dipterocarpacea is ectomycorrhizal, indicating an ancient Gondwanaland origin for the ectomycorrhizal habit in Dipterocarpaceae. New Phytol 172:753–762. CrossRefPubMedGoogle Scholar
  16. Olariaga I, Begoña MJ, García-Etxerbarria K, Salcedo I (2009) Species delimitation in the European species of Clavulina (Cantharellales, Basidiomycota) inferred from phylogenetic analyses of ITS region and morphological data. Mycol Res 113:1261–1270. CrossRefPubMedGoogle Scholar
  17. Olariaga I, Salcedo I (2012) New combinations and notes in clavarioid fungi. Mycotaxon 121:37–44. CrossRefGoogle Scholar
  18. Petersen RH (1964) Notes on Clavarioid Fungi. I. The Clavulina castaneopes complex. Mycologia 56:20–28. CrossRefGoogle Scholar
  19. Petersen RH (1967) Notes of clavarioid fungi VI. Two new species and notes on the origin of Clavulina. Mycologia 59:39–47. CrossRefGoogle Scholar
  20. Petersen RH (1983) Notes on clavarioid fungi XVIII. A preliminary outline of Clavulina in Southeastern Australia. Nova Hedwigia 37:19–35Google Scholar
  21. Petersen RH (1985) Notes on clavarioid fungi XX. New taxa and distributional records in Clavulina and Ramaria. Mycologia 77:903–919. CrossRefGoogle Scholar
  22. Petersen RH (1988a) The clavarioid fungi of New Zealand. DSIR Science Information Publishing, WellingtonGoogle Scholar
  23. Petersen RH (1988b) Notes on clavarioid fungi XXII. Three interesting South American collections. Mycologia 80:571–576. CrossRefGoogle Scholar
  24. Roberts P (1999) Clavarioid fungi from Korup National Park, Cameroon. Kew Bull 54:517–539. CrossRefGoogle Scholar
  25. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574. CrossRefGoogle Scholar
  26. Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (*and other methods) Version 4. Sinauer Associates, SunderlandGoogle Scholar
  27. Tedersoo L, Jairus T, Horton BM, Abarenkov K, Suvi T, Saar I, Kõljalg U (2008) Strong host preference of ectomycorrhizal fungi in a Tasmanian wet sclerophyll forest as revealed by DNA barcoding and taxon-specific primers. New Phytol 180:479–490. CrossRefPubMedGoogle Scholar
  28. Teng SC (1963) Fungi of China. Science Press, BeijingGoogle Scholar
  29. Thacker JR, Henkel TW (2004) New species of Clavulina from Guyana. Mycologia 96:650–657. CrossRefPubMedGoogle Scholar
  30. Thind KS (1961) The Clavariaceae of India. Indian Council of Agricultural Research. New Delhi, IndiaGoogle Scholar
  31. Thind KS, Sharda RM (1984) Three new species of clavarioid fungi from Himalayas. Indian Phytopathology 37:234–240Google Scholar
  32. Trappe JM, Castellano MA (2007) Clavulina lilliputiana, a diminutive new species from Tasmania. Australasian Mycologist 25:87–89Google Scholar
  33. Uehling JK, Henkel TW, Aime MC, Vilgalys R, Smith ME (2012a) New species and distribution records for Clavulina (Cantharellales, Basidiomycota) from the Guiana Shield, with a key to the lowland neotropical taxa. Fungal Biol 116:1263–1274. CrossRefPubMedGoogle Scholar
  34. Uehling JK, Henkel TW, Aime MC, Vilgalys R, Smith ME (2012b) New species of Clavulina with effused or resupinate basidiomata from the Guiana Shield. Mycologia 104:547–556. CrossRefPubMedGoogle Scholar
  35. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246. CrossRefPubMedPubMedCentralGoogle Scholar
  36. Wartchow F (2012) Clavulina incrustata, a new species from Pernambuco, Brazil. Cryptogam Mycol 33:105–113. CrossRefGoogle Scholar
  37. Zang M, Li B, Xi JX (1996) Fungi of the Hengduan Mountains. Science Press, BeijingGoogle Scholar

Copyright information

© German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Life ScienceHunan Normal UniversityChangshaChina

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