Mycological Progress

, Volume 16, Issue 5, pp 535–552 | Cite as

Additions to Lindgomyces (Lindgomycetaceae, Pleosporales, Dothideomycetes), including two new species occurring on submerged wood from North Carolina, USA, with notes on secondary metabolite profiles

  • Huzefa A. RajaEmail author
  • Noemi D. Paguigan
  • Jacques Fournier
  • Nicholas H. Oberlies
Original Article


Two new species of freshwater ascomycetes belonging to the genus Lindgomyces (Pleosporales, Dothideomycetes) are described and illustrated from submerged wood in North Carolina, USA. Lindgomyces carolinensis is characterized by immersed to erumpent ascomata, fissitunicate broadly cylindrical to clavate asci, and fusiform ascospores with acute ends surrounded by a large, fusiform gelatinous sheath. Lindgomyces cigarospora morphologically differs from L. carolinensis in that its ascospores are fusiform to cylindrical with rounded ends, without a large fusiform gelatinous sheath. These two new species nest in the family Lindgomycetaceae based on analyses of combined SSU and LSU rDNA sequence data. Phylogenetic analyses using ITS sequence data support the establishment of the new taxa as separate species within Lindgomyces. In addition to the new species, we report new ITS sequence data for L. cinctosporus and L. griseosporus from France, and L. ingoldianus from North Carolina, USA. We report a video exhibiting fissitunicate ascus dehiscence in L. carolinensis showing ascospore discharge and unraveling of the gelatinous sheath in real time. Chemical analysis of the organic extracts of L. carolinensis and L. cigarospora resulted in two known cyclodepsipeptides, Sch 378161 and Sch 217048. The in situ spatial mapping of these secondary metabolites on fungal cultures indicates the presence of both compounds on the surface of mycelia, as well as being exuded into the surrounding agar.


Freshwater fungi Natural products chemistry Ribosomal genes Systematics 



We thank Dr. J.O. Falkinham, III of Virginia Polytechnic and State University for the antimicrobial testing and Dr. J. Burdette of the University of Illinois at Chicago for the cytotoxicity testing. We also thank Drs. V. Kertesz and G.J. Van Berkel of the Mass Spectrometry and Laser Spectroscopy Group, Chemical Sciences Division (Oak Ridge National Laboratory) for their guidance with the droplet-LMJ-SSP. We thank Dr. A. N Miller, Director of Illinois Natural History Fungarium for providing the ILLS accession numbers for holotype specimens.

Supplementary material

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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Huzefa A. Raja
    • 1
    Email author
  • Noemi D. Paguigan
    • 1
  • Jacques Fournier
    • 2
  • Nicholas H. Oberlies
    • 1
  1. 1.Department of Chemistry and BiochemistryUniversity of North Carolina at GreensboroGreensboroUSA
  2. 2.Las MurosRimontFrance

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