Mycological Progress

, Volume 16, Issue 4, pp 369–391 | Cite as

Clavicipitaceous entomopathogens: new species in Metarhizium and a new genus Nigelia

  • J. Jennifer Luangsa-ard
  • Suchada Mongkolsamrit
  • Donnaya Thanakitpipattana
  • Artit Khonsanit
  • Kanoksri Tasanathai
  • Wasana Noisripoom
  • Richard A. Humber
Original Article


In several surveys in the tropical forests in Thailand, specimens that looked morphologically similar to Metarhizium martiale and Cordyceps variegata, as well as other Metarhizium species were collected and cultured in vitro. A combined phylogeny of several genes including the small (18S) and large (28S) subunits of the ribosomal DNA, elongation factor 1-α (TEF), RNA polymerase II subunits 1 and 2 (RPB1, RPB2) genes has shown these to be new taxa in the Clavicipitaceae. Nigelia is described as a new genus closely related to Metarhizium, to the scale insect pathogens Aschersonia (Hypocrella), Samuelsia and Moelleriella, and to plant pathogens in Claviceps and Balansia, and other relatives. Nigelia comprises M. martiale and a new species Nigelia aurantiaca, which has been found infecting lepidopteran larvae and which produces pseudoimmersed, obliquely arranged, obpyriform perithecia with curved or bent ostioles and with whole (non-separating) cylindric ascospores. Metarhizium chaiyaphumense, M. kalasinense, M. prachinense, M. samlanense, and M. takense are described as new species of Metarhizium. Metarhizium martiale is transferred to Nigelia, and Paecilomyces reniformis is transferred to Metarhizium.


Clavicipitaceae Taxonomy Cordyceps Hypocreales Metarhizium Phylogeny Entomogenous fungi 



The authors would like to thank Drs Lily Eurwilaichitr, Kanyawim Kirtikara (BIOTEC), Prof. Morakot Tanticharoen, and Khun Rungsima Tantalakha for the support of the project. This project was supported by Cluster and Program Management Office grant number P15-51452. Some of the collections were made by Dr Nigel Hywel-Jones, who is thanked for his early contributions to this project. We wish to thank Mr. Rungpet Ridkaew for his earlier contributions to this study. We also wish to thank Prof. Drion Boucias, for sending the Paecilomyces reniformis strains to study. We thank the Thailand Department of National Parks for the permission to collect fungi in the national parks. We are grateful to the anonymous reviewers who made valuable comments and suggestions to improve the manuscript.


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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • J. Jennifer Luangsa-ard
    • 1
  • Suchada Mongkolsamrit
    • 1
  • Donnaya Thanakitpipattana
    • 1
  • Artit Khonsanit
    • 1
  • Kanoksri Tasanathai
    • 1
  • Wasana Noisripoom
    • 1
  • Richard A. Humber
    • 2
  1. 1.Microbe Interaction and Ecology Laboratory (BMIE), BIOTEC, National Science and Technology Development Agency (NSTDA)Khlong LuangThailand
  2. 2.USDA, ARS Biological Integrated Pest Management Research Unit, Robert W. Holley Center for Agriculture and HealthIthacaUSA

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