Mycological Progress

, Volume 15, Issue 10–11, pp 1093–1117 | Cite as

Species diversity of Pseudocercospora from Far East Asia

  • Chiharu Nakashima
  • Keiichi Motohashi
  • Chi-Yu Chen
  • Johannes Z. Groenewald
  • Pedro W. Crous
Original Article


This study reflects on the monophyly of, and species diversity within, the genus Pseudocercospora in Far East Asia. Morphological characteristics and phylogenetic analyses of Pseudocercospora species were based on type specimens and ex-type cultures, which were collected from Japan and Taiwan. A phylogenetic tree was generated from multi-locus DNA sequence data of the internal transcribed spacer regions of the nrDNA cistron (ITS), partial actin (actA), and partial translation elongation factor 1-alpha (tef1), as well as the partial DNA-directed RNA polymerase II second largest subunit (rpb2). Based on these results, Pseudocercospora amelanchieris on Amelanchier and Ps. iwakiensis on Ilex were newly described from Japan, and a further 22 types (incl. two neo-, five lecto-, and 15 epitypes), were designated. The genus Pseudocercospora as presently circumscribed was found to be monophyletic, while the secondary barcodes, actA, tef1, and rpb2 were shown to be well suited to delimitate species within the genus.


Epitypification New species Phylogenetic relationship Species criteria Taxonomy 



This work was supported by grants to the first author by JSPS KAKENHI (grant #17780122, 24780149), JSPS Strategic Young Researcher Overseas Visits Program for Accelerating Brain Circulation (S2507), Institute for fermentation, Osaka and Genebank, National Institute of Agrobiological Science, Japan.

Supplementary material

11557_2016_1231_MOESM1_ESM.pdf (134 kb)
ESM. 1 A subtree extracted from Fig. 1 (PDF 134 kb)


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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Graduate school of BioresourcesMie UniversityMieJapan
  2. 2.Faculty of Regional Environment ScienceTokyo University of AgricultureTokyoJapan
  3. 3.Department of Plant PathologyNational Chung Hsing UniversityTaichungTaiwan
  4. 4.CBS-KNAW Fungal Biodiversity CentreUtrechtThe Netherlands

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