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Mycological Progress

, 15:55 | Cite as

Scolytus multistriatus associated with Dutch elm disease on the island of Gotland: phenology and communities of vectored fungi

  • Audrius Menkis
  • Inga-Lena Östbrant
  • Kateryna Davydenko
  • Remigijus Bakys
  • Maksims Balalaikins
  • Rimvydas Vasaitis
Original Article

Abstract

Scolytus multistriatus Marsham, the smaller European elm bark beetle, is a vector for Dutch elm disease (DED) that in the year 2005 invaded the island of Gotland (Sweden). The island possesses the largest population of elm (mainly Ulmus minor Mill.) in northern Europe. The aim of this study was to monitor flying periods of S. multistriatus during three consecutive years and by using high-throughput sequencing to assess communities of vectored fungi. Sampling of the beetles was carried out at two different sites in Gotland in 2012, 2013, and 2014. In total, 50 pheromone traps were placed at each site and checked weekly during June-August each year. From all sites and years, 177 beetles were trapped. Among these, 6.2 % were trapped in June, 76.8 % in July, and 16.9 % in August (difference significant at p<0.007). Sequencing of ITS rDNA from the beetles revealed the presence of 1589 fungal taxa, among which virulent DED pathogen Ophiostoma novo-ulmi Brasier was the second most common species (9.0 % of all fungal sequences). O. ulmi Buisman, the less virulent DED pathogen, was also detected but only in a single beetle, which was sampled in 2012 (0.04 % of sequences). There were 13.0 % of the beetles infested with O. novo-ulmi in 2012, 4.0 % in 2013, and 27.7 % in 2014. O. novo-ulmi comprised 0.8 % of fungal sequences in 2012, 0.002 % in 2013, and 8.2 % in 2014. The study showed that the proportion of S. multistriatus vectoring O. novo-ulmi has increased in recent years.

Keywords

Ophiostoma Invasive pathogens Bark beetles Disease management Fungal community Ulmus 

Notes

Acknowledgments

We thank Diem Nguyen at the Dept. of Forest Mycology and Plant Pathology, SLU, for language revision and Karin Wågström at the Swedish Forest Agency for help with the field work. The financial support is gratefully acknowledged from Foundation Oscar and Lili Lamms Minne, Carl Tryggers Foundation, the Swedish Research Council Formas, and the EU Life+ Nature Elmias (LIFE12 NAT/SE/001139) project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

11557_2016_1199_MOESM1_ESM.pdf (472 kb)
Supplementary Table 1 (PDF 472 kb)

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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Audrius Menkis
    • 1
  • Inga-Lena Östbrant
    • 2
  • Kateryna Davydenko
    • 3
  • Remigijus Bakys
    • 4
  • Maksims Balalaikins
    • 5
  • Rimvydas Vasaitis
    • 1
  1. 1.Department of Forest Mycology and Plant Pathology, Uppsala BioCenterSwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Swedish Forest Agency Gotland DistrictVisbySweden
  3. 3.Ukrainian Research Institute of Forestry and Forest MeliorationKharkivUkraine
  4. 4.Institute of Forest Biology and SilvicultureAleksandras Stulginskis UniversityAkademija Kaunas DistrictLithuania
  5. 5.Institute of Life Sciences and TechnologyDaugavpils UniversityDaugavpilsLatvia

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