Advertisement

Mycological Progress

, 15:49 | Cite as

Taxonomy and phylogenetic position of Phyllactinia takamatsui, a newly described powdery mildew on cotoneaster, based on molecular and morphological data

  • Seyed Akbar KhodaparastEmail author
  • Amir Hossein Mohammadi
  • Masoumeh Haghdel
  • Hossein Masigol
Original Article
  • 228 Downloads

Abstract

The newly recognised powdery mildew species Phyllactinia takamatsui on Cotoneaster nummularius (Rosaceae) is described and illustrated. This species, collected in Kerman Province, Iran, is well characterised by its conidial morphology and rDNA ITS sequences clearly different from allied species. Conidia are broadly ellipsoid to subcylindrical, i.e. they are not clavate-spathuliform as in most Phyllactinia species. The rDNA ITS sequence analysis showed that this species is closely allied to other species described on hosts belonging to Rosaceae, such as Ph. mali and Ph. pyri-serotinae. The ITS sequence of P. takamatsui was 92 to 94 % similar to that of the closest known relatives. The new species is described in detail, illustrated and compared with other similar taxa.

Keywords

Biodiversity Erysiphales Phyllactinia mali rDNA Rosaceae 

Notes

Acknowledgements

The authors would like to thank the two anonymous referees for their valuable comments that greatly improved the final version of the paper. This work was supported in part by a grant from the Deputy of Research and Technology of the University of Guilan, Iran.

References

  1. Braun U, Cook RTA (2012) Taxonomic manual of the Erysiphales (powdery mildews). CBS Biodivers Ser 11:1–707Google Scholar
  2. Braun U, Paul YS (2009) The Indian Erysiphaceae revisited. Nova Hedwigia 89(3–4):371–395CrossRefGoogle Scholar
  3. Cabrera MG, Vobis G (2011) Phyllactinia sebastianiae sp. nov. on Sebastiania brasiliensis. Mycotaxon 115(1):53–63CrossRefGoogle Scholar
  4. Ershad D (2009) Fungi of Iran. Iranian Research Institute of Plant Protection, TehranGoogle Scholar
  5. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefGoogle Scholar
  6. Havrylenko M (1995) New records of Erysiphaceae from North-Patagonia (Argentina). Nova Hedwigia 61(3–4):447–455Google Scholar
  7. Havrylenko M, Takamatsu S, Divarangkoon R, Braun U (2006) Phyllactinia chubutiana: a new species of erysiphales from Patagonia (Argentina). Mycoscience 47(5):237–241CrossRefGoogle Scholar
  8. Hirata T, Takamatsu S (1996) Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37:265–270CrossRefGoogle Scholar
  9. Khodaparast SA, Takamatsu S, Hedjaroude GA (2001) Phylogenetic structure of the genus Leveillula (Erysiphales: Erysiphaceae) inferred from the nucleotide sequences of the rDNA ITS region with special reference to the L. taurica species complex. Mycol Res 105:909–918CrossRefGoogle Scholar
  10. Khodaparast SA, Niinomi S, Takamatsu S (2007) Molecular and morphological characterization of Leveillula (Ascomycota: Erysiphales) on monocotyledonous plants. Mycol Res 111(6):673–679CrossRefPubMedGoogle Scholar
  11. Khodaparast SA, Takamatsu S, Harada M, Abbasi M, Samadi S (2012) Additional rDNA ITS sequences and its phylogenetic consequences for the genus Leveillula with emphasis on conidium morphology. Mycol Prog 11:741–752CrossRefGoogle Scholar
  12. Takamatsu S, Inagaki M, Niinomi S, Khodaparast SA, Shin HD, Grigaliunaite B, Havrylenko M (2008) Comprehensive molecular phylogenetic analysis and evolution of the genus Phyllactinia (Ascomycota: Erysiphales) and its allied genera. Mycol Res 112(3):299–315CrossRefPubMedGoogle Scholar
  13. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  14. Voytyuk SO, Heluta VP, Wasser SP, Nevo E, Takamatsu S (2009) Biodiversity of the powdery mildew fungi (Erysiphales, Ascomycota) of Israel. Koeltz Scientific BooksGoogle Scholar
  15. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513PubMedGoogle Scholar
  16. White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322Google Scholar

Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Seyed Akbar Khodaparast
    • 1
    Email author
  • Amir Hossein Mohammadi
    • 2
  • Masoumeh Haghdel
    • 2
  • Hossein Masigol
    • 1
  1. 1.Department of Plant Protection, Faculty of Agricultural SciencesUniversity of GuilanRashtIran
  2. 2.Horticultural Science Research Institute, Pistachio Research CenterAgricultural Research, Education and Extension Organization (AREEO)RafsanjanIran

Personalised recommendations