Mycological Progress

, 15:37

Genetic diversity and population structure of Chinese Lentinula edodes revealed by InDel and SSR markers

  • Xingjie Xiang
  • Chuang Li
  • Lei Li
  • Yingbing Bian
  • Hoi Shan Kwan
  • Wenyan Nong
  • Man Kit Cheung
  • Yang Xiao
Original Article

DOI: 10.1007/s11557-016-1183-y

Cite this article as:
Xiang, X., Li, C., Li, L. et al. Mycol Progress (2016) 15: 37. doi:10.1007/s11557-016-1183-y

Abstract

Genetic diversity and population structure of 88 Chinese Lentinula edodes strains belonging to four geographic populations were inferred from 68 Insertion-Deletion (InDel) and two simple sequence repeat (SSR) markers. The overall values of Shannon’s information index and gene diversity were 0.836 and 0.435, respectively, demonstrating a high genetic diversity in Chinese L. edodes strains. Among the four geographic populations, the Central China population displayed a lower genetic diversity. Multiple analyses resolved two unambiguous genetic groups that corresponded to two regions from which the samples were collected—one was a high-altitude region (region 1) and the other was a low-altitude region (region 2). Results from analysis of molecular variance suggested that the majority of genetic variation was contained within populations (74.8 %). Although there was a strong genetic differentiation between populations (FST = 0.252), the variability of ITS sequences from representative strains of the two regions (<3 %) could not support the existence of cryptic species. Pairwise FST values and Nei’s genetic distances showed that there were relatively lower genetic differentiations and genetic distances between populations from the same region. Geographic distribution could play a vital role in the formation of the observed population structure. Mycelium growth rate and precocity of L. edodes strains displayed significant differences between the two regions. Strains from region 2 grew faster and fructified earlier, which could be a result of adaptation to local environmental factors. To the best of our knowledge, this was the first study on the genetic structure and differentiation between populations, as well as the relationship between genetic structure and phenotypic traits in L. edodes.

Keywords

Shiitake mushroom Population genetics InDel markers Mycelium growth rate Precocity 

Supplementary material

11557_2016_1183_MOESM1_ESM.docx (22 kb)
ESM 1(DOCX 22 kb)
11557_2016_1183_MOESM2_ESM.docx (30 kb)
ESM 2(DOCX 29 kb)

Funding information

Funder NameGrant NumberFunding Note
National Natural Science Foundation of China
  • 31372117,
National Key Technology Support Program in the 12th Five-Year Plan of China
  • 2013BAD16B02,
Fundamental Research Funds for the Central Universities of China
  • 2012ZYTS041

Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Xingjie Xiang
    • 1
    • 2
  • Chuang Li
    • 1
    • 2
  • Lei Li
    • 3
  • Yingbing Bian
    • 1
    • 2
  • Hoi Shan Kwan
    • 3
  • Wenyan Nong
    • 3
  • Man Kit Cheung
    • 3
  • Yang Xiao
    • 1
    • 2
  1. 1.Key Laboratory of Agro-Microbial Resource and Development (Ministry of Agriculture)Huazhong Agricultural UniversityHubei ProvincePeople’s Republic of China
  2. 2.Institute of Applied MycologyHuazhong Agricultural UniversityHubei ProvincePeople’s Republic of China
  3. 3.School of Life SciencesThe Chinese University of Hong KongHong Kong SARPeople’s Republic of China

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