Mycological Progress

, 15:29 | Cite as

Venturia orni sp. nov., a species distinct from Venturia fraxini, living in the leaves of Fraxinus ornus

  • Mohammed Ibrahim
  • Markus Schlegel
  • Thomas N. Sieber
Original Article
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Accepted:

Abstract

Venturia fraxini Aderh. s.l. is an endophytic fungus in the leaves of different ash species like Fraxinus excelsior and F. ornus. This study shows that the Venturia species on F. ornus is not conspecific with V. fraxini s.s., but is a closely related species which is described as a new species: Venturia orni sp. nov. The two species distinctly differ morphologically and genetically. In contrast to V. fraxini, V. orni constantly lacks setae lining the ostiolum of the pseudothecia. In addition, the two species differ significantly and constantly in the sequences of the internal transcribed spacer (ITS) region and the genes coding for calmodulin, elongation factor EF-1α, and β-tubulin. Using the same molecular markers, another unknown endophytic Venturia species was discovered on F. ornus. However, this species never sporulated and, thus, a description of its morphology was not possible. Venturia fraxini and V. orni are host-specific and infect only F. excelsior and F. ornus, respectively, although the two hosts are sympatric at all examined sites. Venturia orni is abundant and exhibits high genetic and morphological variability, also in F. ornus planted as ornamentals outside its native range. This study indicates that Fraxinus spp. host an undiscovered wealth of fungal diversity.

Keywords

Venturia Fusicladium Fraxinus Fungal diversity Ash dieback Emerald ash borer 
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Notes

Acknowledgments

We would like to thank to Andrin Gross for his help with the phylogenetic analyses and the Genetic Diversity Center of ETH Zurich for providing laboratory facilities. This work was supported by SNF grant 31003A-146591/1 from the Swiss National Science Foundation, Bern, Switzerland.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Mohammed Ibrahim
    • 1
  • Markus Schlegel
    • 1
  • Thomas N. Sieber
    • 1
  1. 1.Department of Environmental Systems Science, Institute of Integrative Biology, Forest Pathology and DendrologyETH ZurichZurichSwitzerland

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