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Mycological Progress

, 14:106 | Cite as

Identification of genes differentially expressed during the interaction between the plant symbiont Suillus luteus and two plant pathogenic allopatric Heterobasidion species

  • F. Sillo
  • E. Zampieri
  • L. Giordano
  • G. Lione
  • J. V. Colpaert
  • R. Balestrini
  • P. GonthierEmail author
Original Article

Abstract

The effects of biological invasions by non-native species have been widely studied in terms of environmental, economic, and human health impacts. However, little is known about the consequences that non-native plant pathogens may determine on host plant symbionts, such as ectomycorrhizal (ECM) fungi. In this study, interactions between Suillus luteus, an ECM fungus of pine trees, and the allopatrically differentiated fungal pathogens of pines Heterobasidion irregulare and H. annosum were investigated in dual culture by morphological and gene expression analyses. Growth of S. luteus was inhibited by both Heterobasidion species, but based on statistical analysis, growth inhibition was due to the isolate rather than to the species. The expression analysis on genes related to cell wall hydrolytic enzymes and hydrophobins, putatively involved in the fungus–fungus interaction, allowed to identify significantly up- and down-regulated genes both in the symbiont and in the pathogens. Based on the transcript analysis, it was not possible to distinguish the impact of the two pathogenic species on the ECM fungus. The only exception was a S. luteus gene coding for a putative chitinase (SlGH18_8356) that was found to be differentially regulated during interaction with H. irregulare compared to H. annosum.

Keywords

Ectomycorrhizal fungus Fungal pathogen Dual culture Gene expression Cell wall Phylogeny 

Notes

Acknowledgments

This work was supported by the Italian Ministry of Education, University and Research, within the FIRB program (grant number RBFRI280NN).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11557_2015_1130_MOESM1_ESM.doc (237 kb)
ESM 1 (DOC 237 kb)

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Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • F. Sillo
    • 1
  • E. Zampieri
    • 1
  • L. Giordano
    • 1
  • G. Lione
    • 1
  • J. V. Colpaert
    • 2
  • R. Balestrini
    • 3
  • P. Gonthier
    • 1
    Email author
  1. 1.Department of Agricultural, Forest and Food Sciences (DISAFA)University of TorinoGrugliascoItaly
  2. 2.Centre for Environmental Sciences (CMK)Hasselt UniversityDiepenbeekBelgium
  3. 3.Institute for Sustainable Plant ProtectionCNRTorinoItaly

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