Identification of genes differentially expressed during the interaction between the plant symbiont Suillus luteus and two plant pathogenic allopatric Heterobasidion species
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The effects of biological invasions by non-native species have been widely studied in terms of environmental, economic, and human health impacts. However, little is known about the consequences that non-native plant pathogens may determine on host plant symbionts, such as ectomycorrhizal (ECM) fungi. In this study, interactions between Suillus luteus, an ECM fungus of pine trees, and the allopatrically differentiated fungal pathogens of pines Heterobasidion irregulare and H. annosum were investigated in dual culture by morphological and gene expression analyses. Growth of S. luteus was inhibited by both Heterobasidion species, but based on statistical analysis, growth inhibition was due to the isolate rather than to the species. The expression analysis on genes related to cell wall hydrolytic enzymes and hydrophobins, putatively involved in the fungus–fungus interaction, allowed to identify significantly up- and down-regulated genes both in the symbiont and in the pathogens. Based on the transcript analysis, it was not possible to distinguish the impact of the two pathogenic species on the ECM fungus. The only exception was a S. luteus gene coding for a putative chitinase (SlGH18_8356) that was found to be differentially regulated during interaction with H. irregulare compared to H. annosum.
KeywordsEctomycorrhizal fungus Fungal pathogen Dual culture Gene expression Cell wall Phylogeny
This work was supported by the Italian Ministry of Education, University and Research, within the FIRB program (grant number RBFRI280NN).
Conflict of interest
The authors declare that they have no conflict of interest.
- Crawley MJ (2013) The R book, 2nd edn. Wiley, ChichesterGoogle Scholar
- Kéry M (2010) Introduction to WinBUGS for ecologists: a Bayesian approach to regression, ANOVA, mixed models and related analysis. Academic Press, BurlingtonGoogle Scholar
- Kohler A, Kuo A, Nagy LG, Morin E, Barry KW, Buscot F, Canbäck B, Choi C, Cichocki N, Clum A, Colpaert J, Copeland A, Costa MD, Doré J, Floudas D, Gay G, Girlanda M, Henrissat B, Herrmann S, Hess J, Högberg N, Johansson T, Khouja HR, LaButti K, Lahrmann U, Levasseur A, Lindquist EA, Lipzen A, Marmeisse R, Martino E, Murat C, Ngan CY, Nehls U, Plett JM, Pringle A, Ohm RA, Perotto S, Peter M, Riley R, Rineau F, Ruytinx J, Salamov A, Shah F, Sun H, Tarkka M, Tritt A, Veneault-Fourrey C, Zuccaro A; Mycorrhizal Genomics Initiative Consortium, Tunlid A, Grigoriev IV, Hibbett DS, Martin F (2015) Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists. Nat Genet 47:410–415CrossRefPubMedGoogle Scholar
- Lei ZP, Jin JR, Wang CW (1995) Antagonism between ectomycorrhizal fungi and plant pathogens. In: Brundett M, Dell B, Malajczuk M (ed) Mycorrhizas for plantation forestry in Asia. Proceedings of an International Symposium and Workshop, 7–11 November 1994, Kaiping, China, proceedings no. 62. Australian Centre for International Agricultural Research, Canberra, Australia, pp 77–81Google Scholar
- Mohan V, Nivea R, Menon S (2015) Evaluation of ectomycorrhizal fungi as potential bio-control agents against selected plant pathogenic fungi. JAIR 3:408–412Google Scholar
- Napierała-Filipiak A, Werner A (2000) Antagonism of higher fungi to Heterobasidion annosum (Fr.) Bref. in laboratory conditions. Dendrobiology 45:65–81Google Scholar
- Ohm RA, de Jong JF, Lugones LG, Aerts A, Kothe E, Stajich JE, de Vries RP, Record E, Levasseur A, Baker SE, Bartholomew KA, Coutinho PM, Erdmann S, Fowler TJ, Gathman AC, Lombard V, Henrissat B, Knabe N, Kües U, Lilly WW, Lindquist E, Lucas S, Magnuson JK, Piumi F, Raudaskoski M, Salamov A, Schmutz J, Schwarze FW, vanKuyk PA, Horton JS, Grigoriev IV, Wösten HA (2010) Genome sequence of the model mushroom Schizophyllum commune. Nat Biotechnol 28:957–963CrossRefPubMedGoogle Scholar
- Olson A, Aerts A, Asiegbu F, Belbahri L, Bouzid O, Broberg A, Canbäck B, Coutinho PM, Cullen D, Dalman K, Deflorio G, van Diepen LT, Dunand C, Duplessis S, Durling M, Gonthier P, Grimwood J, Fossdal CG, Hansson D, Henrissat B, Hietala A, Himmelstrand K, Hoffmeister D, Högberg N, James TY, Karlsson M, Kohler A, Kües U, Lee YH, Lin YC, Lind M, Lindquist E, Lombard V, Lucas S, Lundén K, Morin E, Murat C, Park J, Raffaello T, Rouzé P, Salamov A, Schmutz J, Solheim H, Ståhlberg J, Vélëz H, de Vries RP, Wiebenga A, Woodward S, Yakovlev I, Garbelotto M, Martin F, Grigoriev IV, Stenlid J (2012) Insight into trade-off between wood decay and parasitism from the genome of a fungal forest pathogen. New Phytol 194:1001–1013CrossRefPubMedGoogle Scholar
- Sivan A, Chet I (1989) Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. J Gen Microbiol 135:675–682Google Scholar
- Yakovlev I, Vaaje-Kolstad G, Hietala AM, Stefańczyk E, Solheim H, Fossdal CG (2012) Substrate-specific transcription of the enigmatic GH61 family of the pathogenic white-rot fungus Heterobasidion irregulare during growth on lignocellulose. Appl Microbiol Biotechnol 95:979–990PubMedCentralCrossRefPubMedGoogle Scholar